alpha 1 Antitrypsin antibody [9A1]

Cat. No. GTX83666
1
Cat. No.  GTX83666
KO/KD Overexpression
Anti-alpha 1 Antitrypsin antibody [9A1] used in Flow cytometry (FCM). GTX83666
GTX83666 FCM Image

FACS analysis of HEK293T cells transfected with either alpha 1 Antitrypsin plasmid(Red) or empty vector control plasmid(Blue) using GTX83666 alpha 1 Antitrypsin antibody [9A1].

1 / 11
Anti-alpha 1 Antitrypsin antibody [9A1] used in IHC (Paraffin sections) (IHC-P). GTX83666
GTX83666 IHC-P Image

IHC-P analysis of human endometrium adenocarcinoma tissue using GTX83666 alpha 1 Antitrypsin antibody [9A1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

2 / 11
Anti-alpha 1 Antitrypsin antibody [9A1] used in IHC (Paraffin sections) (IHC-P). GTX83666
GTX83666 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX83666 alpha 1 Antitrypsin antibody [9A1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

3 / 11
Anti-alpha 1 Antitrypsin antibody [9A1] used in Western Blot (WB). GTX83666
GTX83666 WB Image

WB analysis of human tissues (Lane 1-Uterus ; Lane 2-Breast ; Lane 3-Brain ; Lane 4-Liver ; Lane 5-Ovary ; Lane 6-Thyroid gland ; Lane 7-colon) using GTX83666 alpha 1 Antitrypsin antibody [9A1].
Loading : 10 ug per lane
Dilution : 1:200

4 / 11
Anti-alpha 1 Antitrypsin antibody [9A1] used in Western Blot (WB). GTX83666
GTX83666 WB Image

WB analysis of various cell lines using GTX83666 alpha 1 Antitrypsin antibody [9A1].
Loading : 35 ug per lane

5 / 11
Anti-alpha 1 Antitrypsin antibody [9A1] used in IHC (Paraffin sections) (IHC-P). GTX83666
GTX83666 IHC-P Image

IHC-P analysis of human liver carcinoma tissue using GTX83666 alpha 1 Antitrypsin antibody [9A1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

6 / 11
Anti-alpha 1 Antitrypsin antibody [9A1] used in Immunoprecipitation (IP). GTX83666
GTX83666 IP Image

IP analysis of DDDDK tagged alpha 1 Antitrypsin overexpressed HEK293T lysate using GTX83666 alpha 1 Antitrypsin antibody [9A1].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

7 / 11
Anti-alpha 1 Antitrypsin antibody [9A1] used in Western Blot (WB). GTX83666
GTX83666 WB Image
Overexpression

WB analysis of HEK293T cells transfected with alpha 1 Antitrypsin plasmid (Right) or empty vector (Left) for 48 hrs using GTX83666 alpha 1 Antitrypsin antibody [9A1].
Loading : 5 ug per lane

8 / 11
Anti-alpha 1 Antitrypsin antibody [9A1] used in IHC (Paraffin sections) (IHC-P). GTX83666
GTX83666 IHC-P Image

IHC-P analysis of human liver tissue using GTX83666 alpha 1 Antitrypsin antibody [9A1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

9 / 11
Anti-alpha 1 Antitrypsin antibody [9A1] used in IHC (Paraffin sections) (IHC-P). GTX83666
GTX83666 IHC-P Image

IHC-P analysis of human thyroid carcinoma tissue using GTX83666 alpha 1 Antitrypsin antibody [9A1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

10 / 11
Anti-alpha 1 Antitrypsin antibody [9A1] used in IHC (Frozen sections) (IHC-Fr). GTX83666
GTX83666 IHC-Fr Image
KO/KD

The data was published in the 2022 in JCI Insight. PMID: 35104244

11 / 11
Anti-alpha 1 Antitrypsin antibody [9A1] used in Flow cytometry (FCM). GTX83666
Anti-alpha 1 Antitrypsin antibody [9A1] used in IHC (Paraffin sections) (IHC-P). GTX83666
Anti-alpha 1 Antitrypsin antibody [9A1] used in IHC (Paraffin sections) (IHC-P). GTX83666
Anti-alpha 1 Antitrypsin antibody [9A1] used in Western Blot (WB). GTX83666
Anti-alpha 1 Antitrypsin antibody [9A1] used in Western Blot (WB). GTX83666
Anti-alpha 1 Antitrypsin antibody [9A1] used in IHC (Paraffin sections) (IHC-P). GTX83666
Anti-alpha 1 Antitrypsin antibody [9A1] used in Immunoprecipitation (IP). GTX83666
Anti-alpha 1 Antitrypsin antibody [9A1] used in Western Blot (WB). GTX83666
Anti-alpha 1 Antitrypsin antibody [9A1] used in IHC (Paraffin sections) (IHC-P). GTX83666
Anti-alpha 1 Antitrypsin antibody [9A1] used in IHC (Paraffin sections) (IHC-P). GTX83666
Anti-alpha 1 Antitrypsin antibody [9A1] used in IHC (Frozen sections) (IHC-Fr). GTX83666
This alpha 1 Antitrypsin antibody [9A1] (GTX83666) is a mouse monoclonal antibody validated for detection of alpha 1 Antitrypsin by WB, IHC-P, IHC-Fr, FCM, and IP. Reactive to human, dog, monkey, and other species. Knockout/knockdown validation supports antibody specificity.
  • Host
    Mouse
  • Clonality
    Monoclonal
  • Clone Name
    9A1
  • Isotype
    IgG1
  • Applications
    WB IHC-P IHC-Fr FCM IP
  • Reactivity
    Human, Dog, Monkey, Ferret
See all alpha 1 Antitrypsin products

Ask