alpha 1 Antitrypsin antibody [9A1]

Name: alpha 1 Antitrypsin antibody [9A1]
Brand: GeneTex
SKU: GTX83666
Price: 519.00 USD

Anti-alpha 1 Antitrypsin antibody [9A1] used in IHC (Paraffin sections) (IHC-P). GTX83666

Anti-alpha 1 Antitrypsin antibody [9A1] used in Western Blot (WB). GTX83666

Anti-alpha 1 Antitrypsin antibody [9A1] used in Immunoprecipitation (IP). GTX83666

Anti-alpha 1 Antitrypsin antibody [9A1] used in Flow cytometry (FACS). GTX83666

Anti-alpha 1 Antitrypsin antibody [9A1] used in IHC (Frozen sections) (IHC-Fr). GTX83666

Cat. No. GTX83666

Reference 1

Host	Mouse
Clonality	Monoclonal
Clone Name	9A1
Isotype	IgG1
Application	WB, IHC-P, IHC-Fr, FACS, IP
Reactivity	Human, Dog, Monkey, Ferret
Package	100 μl ($519)

See all alpha 1 Antitrypsin products

View product citations for antibody GTX83666 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:1000
IHC-P	1:150
IHC-Fr	Assay dependent
FACS	1:100
IP	2ug/500ul

Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human SERPINA1 expression vector

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

serpin family A member 1 , A1A , A1AT , AAT , PI , PI1 , PRO2275 , alpha1AT , nNIF

Cellular Localization

Secreted,Endoplasmic reticulum

Background

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Short peptide from AAT (SPAAT) is a reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).

Database

Gene ID: 5265 SERPINA1
UniProt: P01009 SERPINA1

Research Area

DATA IMAGES

GTX83666 IHC-P Image

IHC-P analysis of human thyroid carcinoma tissue using GTX83666 alpha 1 Antitrypsin antibody [9A1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83666 IHC-P Image

IHC-P analysis of human liver tissue using GTX83666 alpha 1 Antitrypsin antibody [9A1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83666 WB Image

WB analysis of HEK293T cells transfected with alpha 1 Antitrypsin plasmid (Right) or empty vector (Left) for 48 hrs using GTX83666 alpha 1 Antitrypsin antibody [9A1].
Loading : 5 ug per lane

GTX83666 IP Image

IP analysis of DDDDK tagged alpha 1 Antitrypsin overexpressed HEK293T lysate using GTX83666 alpha 1 Antitrypsin antibody [9A1].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

GTX83666 IHC-P Image

IHC-P analysis of human liver carcinoma tissue using GTX83666 alpha 1 Antitrypsin antibody [9A1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83666 WB Image

WB analysis of various cell lines using GTX83666 alpha 1 Antitrypsin antibody [9A1].
Loading : 35 ug per lane

GTX83666 WB Image

WB analysis of human tissues (Lane 1-Uterus ; Lane 2-Breast ; Lane 3-Brain ; Lane 4-Liver ; Lane 5-Ovary ; Lane 6-Thyroid gland ; Lane 7-colon) using GTX83666 alpha 1 Antitrypsin antibody [9A1].
Loading : 10 ug per lane
Dilution : 1:200

GTX83666 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX83666 alpha 1 Antitrypsin antibody [9A1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83666 IHC-P Image

IHC-P analysis of human endometrium adenocarcinoma tissue using GTX83666 alpha 1 Antitrypsin antibody [9A1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83666 FACS Image

FACS analysis of HEK293T cells transfected with either alpha 1 Antitrypsin plasmid(Red) or empty vector control plasmid(Blue) using GTX83666 alpha 1 Antitrypsin antibody [9A1].

GTX83666 IHC-Fr Image

The data was published in the 2022 in JCI Insight. PMID: 35104244

REFERENCE

Application Reference

He N et al. JCI Insight 2022; (Epub) Ferret models of alpha-1 antitrypsin deficiency develop lung and liver disease.

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REVIEW

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Application Reference

He N et al. JCI Insight 2022; (Epub) Ferret models of alpha-1 antitrypsin deficiency develop lung and liver disease.

SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty

alpha 1 Antitrypsin antibody [9A1]

Cat. No. GTX83666

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX83666 IHC-P Image

GTX83666 IHC-P Image

GTX83666 WB Image

GTX83666 IP Image

GTX83666 IHC-P Image

GTX83666 WB Image

GTX83666 WB Image

GTX83666 IHC-P Image

GTX83666 IHC-P Image

GTX83666 FACS Image

GTX83666 IHC-Fr Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.