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alpha 1 Antitrypsin antibody [AAT/1378]

Anti-alpha 1 Antitrypsin antibody [AAT/1378] used in IHC (Paraffin sections) (IHC-P). GTX34403
Anti-alpha 1 Antitrypsin antibody [AAT/1378] used in Western Blot (WB). GTX34403

Cat No. GTX34403

Host Mouse
Clonality Monoclonal
Clone Name AAT/1378
Isotype IgG1
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1.0ug/ml
ICC/IF 0.5-1ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Specificity/Sensitivity

It recognizes a protein of 54kDa, which is identified antitrypsin (AAT). The immunohistochemical staining of AAT is useful in identification of benign and malignant hepatic tumors and yolk sac carcinomas. Positive staining for AAT is also used in detection of benign and malignant lesions of histiocytic nature. This antibody is may also useful tool in the screening of patients with cryptogenic cirrhosis or other forms of liver disease with fibrosis of uncertain origin.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human Alpha-1-Antitrypsin (AAT) protein fragment (exact sequence is proprietary)

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Serpin Family A Member 1,A1A,A1At,Aat,Pi,Pi1,Pro2275,Alpha1At,Nnif,Serpina1

Background

The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-alpha 1 Antitrypsin antibody [AAT/1378] used in IHC (Paraffin sections) (IHC-P). GTX34403

GTX34403 IHC-P Image

Formalin-fixed, paraffin-embedded human Tonsil stained with Alpha-1-Antitrypsin Monoclonal Antibody (AAT/1378).

Anti-alpha 1 Antitrypsin antibody [AAT/1378] used in Western Blot (WB). GTX34403

GTX34403 WB Image

Western Blot of recombinant Alpha-1-Antitrypsin, Jurkat and A549 Cell Lysate using Alpha-1-Antitrypsin Monoclonal Antibody (AAT/1378).