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alpha 1 Antitrypsin antibody [TMF1#4B5]

Anti-alpha 1 Antitrypsin antibody [TMF1#4B5] used in Western Blot (WB). GTX15777
Anti-alpha 1 Antitrypsin antibody [TMF1#4B5] used in Western Blot (WB). GTX15777
Anti-alpha 1 Antitrypsin antibody [TMF1#4B5] used in Flow cytometry (FACS). GTX15777

Cat. No. GTX15777

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 TMF1#4B5

Isotype

 IgG2a

Application

 WB, FACS, IP, ELISA, RIA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all alpha 1 Antitrypsin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-3 μg/ml
FACS 3-5 μg/10⁶ cells
IP Assay dependent
ELISA Assay dependent
RIA Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM Potassium Phosphate, 2.5mM KCl, 135mM NaCl, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified human a1-Antitrypsin

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

serpin family A member 1 , A1A , A1AT , AAT , PI , PI1 , PRO2275 , alpha1AT , nNIF

Cellular Localization

Secreted,Endoplasmic reticulum

Background

The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-alpha 1 Antitrypsin antibody [TMF1#4B5] used in Western Blot (WB). GTX15777

GTX15777 WB Image

WB analysis of membrane enriched extracts (30 ug) of HT-29 (Lane 1) and Hep G2 (Lane 2) in figure 1, and condition media of Hep G2 (Lane 1), PC3 (Lane 2), HeLa (Lane 3) in figure 2 using GTX15777 alpha 1 Antitrypsin antibody [TMF1#4B5].
Dilution : 2 μg/ml

Anti-alpha 1 Antitrypsin antibody [TMF1#4B5] used in Western Blot (WB). GTX15777

GTX15777 WB Image

WB analysis of membrane enriched extracts (30 ug lysate) of Hep G2 (Lane 1) and tissue extract of Mouse Liver (Lane 2) using GTX15777 alpha 1 Antitrypsin antibody [TMF1#4B5].
Dilution : 2 μg/ml

Anti-alpha 1 Antitrypsin antibody [TMF1#4B5] used in Flow cytometry (FACS). GTX15777

GTX15777 FACS Image

FACS analysis of 70% ethanol fixed HepG2 cells using GTX15777 alpha 1 Antitrypsin antibody [TMF1#4B5].
Red histogram : Primary antibody
Pink histogram : mouse isotype control
Fixation : 70% ethanol for 10 minutes
Permeabilization : 0.25% Trito X-100 for 20 minutes
Dilution : 3-5 μg/million cells in 2.5% BSA for 30 minutes at room temperature

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399