GeneTex
United States (US)

alpha 1 Antitrypsin antibody [TMF1#4B5]

GTX15777_1530_WB_19011519_319.jpg
GTX15777_116_FACS_19011519_496.jpg
GTX15777_1529_WB_19011519_963.jpg

Cat No. GTX15777

Host Mouse
Clonality Monoclonal
Clone Name TMF1#4B5
Isotype IgG2a
Application WB, FACS, IP, ELISA, RIA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3 μg/ml
FACS 3-5 μg/10⁶ cells
IP Assay dependent
ELISA Assay dependent
RIA Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.01M potassium phosphate, 2.5mM KCl, 0.135M NaCl, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified human a1-Antitrypsin

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Serpin Family A Member 1,A1A,A1At,Aat,Pi,Pi1,Pro2275,Alpha1At,Nnif,Serpina1

Cellular Localization

Endoplasmic reticulum,Extracellular matrix,Extracellular space,Secreted

Background

The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX15777_1530_WB_19011519_319.jpg

GTX15777 WB Image

WB analysis of membrane enriched extracts (30 ug) of HT-29 (Lane 1) and Hep G2 (Lane 2) in figure 1, and condition media of Hep G2 (Lane 1), PC3 (Lane 2), HeLa (Lane 3) in figure 2 using GTX15777 alpha 1 Antitrypsin antibody [TMF1#4B5].
Dilution : 2 µg/ml

GTX15777_116_FACS_19011519_496.jpg

GTX15777 FACS Image

FACS analysis of 70% ethanol fixed HepG2 cells using GTX15777 alpha 1 Antitrypsin antibody [TMF1#4B5].
Red histogram : Primary antibody
Pink histogram : mouse isotype control
Fixation : 70% ethanol for 10 minutes
Permeabilization : 0.25% Trito X-100 for 20 minutes
Dilution : 3-5 µg/million cells in 2.5% BSA for 30 minutes at room temperature

GTX15777_1529_WB_19011519_963.jpg

GTX15777 WB Image

WB analysis of membrane enriched extracts (30 ug lysate) of Hep G2 (Lane 1) and tissue extract of Mouse Liver (Lane 2) using GTX15777 alpha 1 Antitrypsin antibody [TMF1#4B5].
Dilution : 2 µg/ml