WB analysis of membrane enriched extracts (30 ug lysate) of Hep G2 (Lane 1) and tissue extract of Mouse Liver (Lane 2) using GTX15777 alpha 1 Antitrypsin antibody [TMF1#4B5].
Dilution : 2 μg/ml

WB analysis of membrane enriched extracts (30 ug) of HT-29 (Lane 1) and Hep G2 (Lane 2) in figure 1, and condition media of Hep G2 (Lane 1), PC3 (Lane 2), HeLa (Lane 3) in figure 2 using GTX15777 alpha 1 Antitrypsin antibody [TMF1#4B5].
Dilution : 2 μg/ml

FACS analysis of 70% ethanol fixed HepG2 cells using GTX15777 alpha 1 Antitrypsin antibody [TMF1#4B5].
Red histogram : Primary antibody
Pink histogram : mouse isotype control
Fixation : 70% ethanol for 10 minutes
Permeabilization : 0.25% Trito X-100 for 20 minutes
Dilution : 3-5 μg/million cells in 2.5% BSA for 30 minutes at room temperature