APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
0.02M Potassium Phosphate pH7.2, 0.15M Sodium Chloride
Preservative
0.01% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
10 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
a1-Anti-Trypsin [Human Plasma]
Purification
IgG fraction
From polyclonal serum
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
serpin family A member 1 , A1A , A1AT , AAT , PI , PI1 , PRO2275 , alpha1AT , nNIF
Cellular Localization
Secreted,Endoplasmic reticulum
Background
alpha 1 Antitrypsin is a plasma protein synthesised in the liver. It consists of a single polypeptide chain and has a molecular weight of 52 kDa. A serine protease inhibitor, its primary function is the protection of lung tissue from the action of neutrophil elastase. The average level of alpha 1 Antitrypsin in plasma is 1.3 g/l.
Database
Research Area
DATA IMAGES
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GTX27633 WB Image
Western Blot of Goat anti Alpha-1anti-Trypsin antibody.
Lane 1: Alpha-1anti-Trypsin under reducing conditions.
Lane 2: none.
Load: 1 µg per lane. Primary antibody: Alpha-1anti-Trypsin antibody (GTX27633) at 1:3000 for overnight at 4°C. Secondary antibody: Flourescent dye 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 45 min at RT. Block: overnight at 4°C. Predicted/Observed size: 55kDa for a-1 Anti-Trypsin. Other band(s): none
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REFERENCE
There are currently no references for alpha 1 Antitrypsin antibody (GTX27633). Be the first to share your publications with this product.
REVIEW
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