alpha 1 Antitrypsin antibody

Cat No. GTX27633

Host	Goat
Clonality	Polyclonal
Isotype	IgG
Application	WB, IP, Dot, ELISA, Conjugation
Reactivity	Human

See all alpha 1 Antitrypsin products

Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	Assay dependent
IP	Assay dependent
Dot	Assay dependent
ELISA	Assay dependent
Conjugation	Assay dependent

Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium phosphate, 0.15 M sodium chloride, pH 7.2, containing 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

10 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

a1-Anti-Trypsin [Human Plasma]

Purification

IgG fraction
From polyclonal serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Serpin Family A Member 1,A1A,A1At,Aat,Pi,Pi1,Pro2275,Alpha1At,Nnif,Serpina1

Cellular Localization

Secreted

Background

alpha 1 Antitrypsin is a plasma protein synthesised in the liver. It consists of a single polypeptide chain and has a molecular weight of 52 kDa. A serine protease inhibitor, its primary function is the protection of lung tissue from the action of neutrophil elastase. The average level of alpha 1 Antitrypsin in plasma is 1.3 g/l.

Database

Gene ID: 5265
UniProt: P01009

Research Area

- Cancer
- Cancer
- Hypoxia

DATA IMAGES

GTX27633 WB Image

Western Blot of Goat anti Alpha-1anti-Trypsin antibody. Lane 1: Alpha-1anti-Trypsin under reducing conditions. Lane 2: none. Load: 1 µg per lane. Primary antibody: Alpha-1anti-Trypsin antibody (GTX27633) at 1:3000 for overnight at 4°C. Secondary antibody: Flourescent dye 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 45 min at RT. Block: overnight at 4°C. Predicted/Observed size: 55kDa for a-1 Anti-Trypsin. Other band(s): none