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alpha 1 Antitrypsin antibody

Anti-alpha 1 Antitrypsin antibody used in Western Blot (WB). GTX27633

Cat No. GTX27633

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP, Dot, ELISA, Conjugation

Reactivity

 Human
Package
1 mg ($299)
See all alpha 1 Antitrypsin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IP Assay dependent
Dot Assay dependent
ELISA Assay dependent
Conjugation Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate pH7.2, 0.15M Sodium Chloride

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

10 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

a1-Anti-Trypsin [Human Plasma]

Purification

IgG fraction
From polyclonal serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

serpin family A member 1 , A1A , A1AT , AAT , PI , PI1 , PRO2275 , alpha1AT , nNIF

Cellular Localization

Secreted,Endoplasmic reticulum

Background

alpha 1 Antitrypsin is a plasma protein synthesised in the liver. It consists of a single polypeptide chain and has a molecular weight of 52 kDa. A serine protease inhibitor, its primary function is the protection of lung tissue from the action of neutrophil elastase. The average level of alpha 1 Antitrypsin in plasma is 1.3 g/l.

Database

Research Area

DATA IMAGES

Anti-alpha 1 Antitrypsin antibody used in Western Blot (WB). GTX27633

GTX27633 WB Image

Western Blot of Goat anti Alpha-1anti-Trypsin antibody. Lane 1: Alpha-1anti-Trypsin under reducing conditions. Lane 2: none. Load: 1 µg per lane. Primary antibody: Alpha-1anti-Trypsin antibody (GTX27633) at 1:3000 for overnight at 4°C. Secondary antibody: Flourescent dye 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 45 min at RT. Block: overnight at 4°C. Predicted/Observed size: 55kDa for a-1 Anti-Trypsin. Other band(s): none

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 299