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alpha 1 Catenin antibody, Internal

Anti-alpha 1 Catenin antibody, Internal used in Western Blot (WB). GTX89347
Anti-alpha 1 Catenin antibody, Internal used in Western Blot (WB). GTX89347
Anti-alpha 1 Catenin antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89347

Cat. No. GTX89347

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all alpha 1 Catenin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-3μg/ml
IHC-P 3-6μg/ml

Note :

IHC-P
Human Prostate shows staining of cell membranes of the secretory cells in the glands.
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89347-PEP).

Predict Reactivity

Mouse, Rat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-KREKQDETQTKIK, from the internal region of the protein sequence according to NP_001894.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

catenin alpha 1 , CAP102 , MDPT2

Cellular Localization

Cell junction

Background

This gene encodes a member of the catenin family of proteins that play an important role in cell adhesion process by connecting cadherins located on the plasma membrane to the actin filaments inside the cell. The encoded mechanosensing protein contains three vinculin homology domains and undergoes conformational changes in response to cytoskeletal tension, resulting in the reconfiguration of cadherin-actin filament connections. Certain mutations in this gene cause butterfly-shaped pigment dystrophy. [provided by RefSeq, May 2016]

Database

Research Area

DATA IMAGES

Anti-alpha 1 Catenin antibody, Internal used in Western Blot (WB). GTX89347

GTX89347 WB Image

WB analysis of HEK293 overexpressing human alpha 1 Catenin (mock transfection in first lane) using GTX89347 alpha 1 Catenin antibody, Internal.

Anti-alpha 1 Catenin antibody, Internal used in Western Blot (WB). GTX89347

GTX89347 WB Image

WB analysis of human colon lysate using GTX89347 alpha 1 Catenin antibody, Internal.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

Anti-alpha 1 Catenin antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89347

GTX89347 IHC-P Image

IHC-P analysis of human prostate using GTX89347 alpha 1 Catenin antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399