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alpha 1 Catenin antibody [N3C2], Internal

Anti-alpha 1 Catenin antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111095
Anti-alpha 1 Catenin antibody [N3C2], Internal used in Western Blot (WB). GTX111095
Anti-alpha 1 Catenin antibody [N3C2], Internal used in Western Blot (WB). GTX111095
Anti-alpha 1 Catenin antibody [N3C2], Internal used in Western Blot (WB). GTX111095
Anti-alpha 1 Catenin antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111095
Anti-alpha 1 Catenin antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111095
Anti-alpha 1 Catenin antibody [N3C2], Internal used in Western Blot (WB). GTX111095
Anti-alpha 1 Catenin antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111095
Anti-alpha 1 Catenin antibody [N3C2], Internal used in Western Blot (WB). GTX111095

Cat. No. GTX111095

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat, Zebrafish, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all alpha 1 Catenin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

NIH-3T3 , JC , PC-12

Predict Reactivity

Rabbit, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.87 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha 1 Catenin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950058

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

catenin alpha 1 , CAP102 , MDPT2

Cellular Localization

Cytoplasm , cytoskeleton , Cell junction , adherens junction , Cell membrane

Background

Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherence junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.

Database

Research Area

DATA IMAGES

Anti-alpha 1 Catenin antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111095

GTX111095 IHC-P Image

alpha 1 Catenin antibody [N3C2], Internal detects alpha 1 Catenin protein at membrane on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
alpha 1 Catenin antibody [N3C2], Internal (GTX111095) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha 1 Catenin antibody [N3C2], Internal used in Western Blot (WB). GTX111095

GTX111095 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
7.5% SDS PAGE
GTX111095 diluted at 1:10000

Anti-alpha 1 Catenin antibody [N3C2], Internal used in Western Blot (WB). GTX111095

GTX111095 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha 1 Catenin antibody (GTX111095) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha 1 Catenin antibody [N3C2], Internal used in Western Blot (WB). GTX111095

GTX111095 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha 1 Catenin antibody (GTX111095) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha 1 Catenin antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111095

GTX111095 ICC/IF Image

alpha 1 catenin antibody [N3c2], Internal detects alpha 1 Catenin protein at cytoplasm and cell junction by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha 1 Catenin protein stained by alpha 1 catenin antibody [N3c2], Internal (GTX111095) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-alpha 1 Catenin antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111095

GTX111095 ICC/IF Image

alpha 1 Catenin antibody [N3C2], Internal detects alpha 1 Catenin protein at cell junction by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha 1 Catenin stained by alpha 1 Catenin antibody [N3C2], Internal (GTX111095) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-alpha 1 Catenin antibody [N3C2], Internal used in Western Blot (WB). GTX111095

GTX111095 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha 1 Catenin antibody [N3C2], Internal (GTX111095) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha 1 Catenin antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111095

GTX111095 IHC-P Image

alpha 1 Catenin antibody [N3C2], Internal detects alpha 1 Catenin protein at membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
alpha 1 Catenin antibody [N3C2], Internal (GTX111095) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha 1 Catenin antibody [N3C2], Internal used in Western Blot (WB). GTX111095

GTX111095 WB Image

Sample (30 ug of whole cell lysate)
A: PC-12
7.5% SDS PAGE
GTX111095 diluted at 1:10000

REFERENCE

Application Reference

Li X et al. Mol Cell 2018; 72 (4) : 650-660.e8 Nuclear PGK1 Alleviates ADP-Dependent Inhibition of CDC7 to Promote DNA Replication.
Application : WB
Reactivity : Mouse
Stewart S et al. Cell Rep 2014; 6 (3) : 482-98 Sequential and opposing activities of Wnt and BMP coordinate zebrafish bone regeneration.
Application : IHC-Fr
Reactivity : Zebrafish
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169