GeneTex
United States (US)

alpha 1 Catenin antibody

GTX106014_39953_20160310_WB_R_brain_19011506_888.jpg
GTX106014_39953_WB_M_brain_19011506_571.jpg
GTX106014_WB_19011506_281.jpg
GTX106014_39953_IHC_19011506_607.jpg

Cat No. GTX106014

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

293T , A431 , mouse brain , rat brain

Predict Reactivity

Zebrafish, Rabbit, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha 1 Catenin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CAP102 antibody, FLJ36832 antibody, FLJ52416 antibody, CTNNA1 antibody, "cadherin-associated protein,102kDa antibody", alpha-catenin antibody, alphaE-catenin antibody, catenin alpha-1 antibody, renal carcinoma antigen NY-REN-13 antibody, alpha-E-catenin antibody, alpha E-catenin antibody, "catenin (cadherin-associated protein), alpha 1, 102kDa antibody", alpha catenin antibody, alpha catenin

Cellular Localization

Cytoplasm , cytoskeleton , Cell junction , adherens junction , Cell membrane

Background

Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherence junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.

Database

Research Area

DATA IMAGES
GTX106014_39953_20160310_WB_R_brain_19011506_888.jpg

GTX106014 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Catenin antibody (GTX106014) diluted at 1:500.

GTX106014_39953_WB_M_brain_19011506_571.jpg

GTX106014 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX106014 diluted at 1:1000

GTX106014_WB_19011506_281.jpg

GTX106014 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
GTX106014 diluted at 1:5000

GTX106014_39953_IHC_19011506_607.jpg

GTX106014 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using alpha Catenin(GTX106014) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169