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alpha 1 Catenin antibody

KO/KD
Anti-alpha 1 Catenin antibody used in Western Blot (WB). GTX32433
Anti-alpha 1 Catenin antibody used in IHC (Paraffin sections) (IHC-P). GTX32433
Anti-alpha 1 Catenin antibody used in Immunoprecipitation (IP). GTX32433
Anti-alpha 1 Catenin antibody used in IHC (Paraffin sections) (IHC-P). GTX32433
Anti-alpha 1 Catenin antibody used in IHC (Paraffin sections) (IHC-P). GTX32433
Anti-alpha 1 Catenin antibody used in Western Blot (WB). GTX32433
Anti-alpha 1 Catenin antibody used in IHC (Paraffin sections) (IHC-P). GTX32433

Cat. No. GTX32433

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all alpha 1 Catenin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
IP 1:50 - 1:100
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 637-906 of human CTNNA1 (NP_001894.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

catenin alpha 1 , CAP102 , MDPT2

Cellular Localization

Cell junction

Background

This gene encodes a member of the catenin family of proteins that play an important role in cell adhesion process by connecting cadherins located on the plasma membrane to the actin filaments inside the cell. The encoded mechanosensing protein contains three vinculin homology domains and undergoes conformational changes in response to cytoskeletal tension, resulting in the reconfiguration of cadherin-actin filament connections. Certain mutations in this gene cause butterfly-shaped pigment dystrophy. [provided by RefSeq, May 2016]

Database

Research Area

DATA IMAGES

Anti-alpha 1 Catenin antibody used in Western Blot (WB). GTX32433

GTX32433 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX32433 alpha 1 Catenin antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-alpha 1 Catenin antibody used in IHC (Paraffin sections) (IHC-P). GTX32433

GTX32433 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX32433 alpha 1 Catenin antibody.
Dilution : 1:100

Anti-alpha 1 Catenin antibody used in Immunoprecipitation (IP). GTX32433

GTX32433 IP Image

IP analysis of 293T cell lysate using GTX32433 alpha 1 Catenin antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:500

Anti-alpha 1 Catenin antibody used in IHC (Paraffin sections) (IHC-P). GTX32433

GTX32433 IHC-P Image

IHC-P analysis of human stomach tissue using GTX32433 alpha 1 Catenin antibody.
Dilution : 1:100

Anti-alpha 1 Catenin antibody used in IHC (Paraffin sections) (IHC-P). GTX32433

GTX32433 IHC-P Image

IHC-P analysis of human vermiform appendix tissue using GTX32433 alpha 1 Catenin antibody.
Dilution : 1:100

Anti-alpha 1 Catenin antibody used in Western Blot (WB). GTX32433

GTX32433 WB Image

WB analysis of various sample lysates using GTX32433 alpha 1 Catenin antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-alpha 1 Catenin antibody used in IHC (Paraffin sections) (IHC-P). GTX32433

GTX32433 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX32433 alpha 1 Catenin antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399