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alpha 1 Spectrin antibody [GT1313]

Recombinant antibody Orthogonal
Anti-alpha 1 Spectrin antibody [GT1313] used in Western Blot (WB). GTX03225
Anti-alpha 1 Spectrin antibody [GT1313] used in Western Blot (WB). GTX03225

Cat. No. GTX03225

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1313

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($439)
See all alpha 1 Spectrin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

280 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.05 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human alpha 1 Spectrin.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

spectrin alpha, erythrocytic 1 , EL2 , HPP , HS3 , SPH3 , SPTA

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene encodes a member of a family of molecular scaffold proteins that link the plasma membrane to the actin cytoskeleton and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. The encoded protein is primarily composed of 22 spectrin repeats which are involved in dimer formation. It forms a component of the erythrocyte plasma membrane. Mutations in this gene result in a variety of hereditary red blood cell disorders, including elliptocytosis-2, pyropoikilocytosis, and spherocytosis, type 3. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-alpha 1 Spectrin antibody [GT1313] used in Western Blot (WB). GTX03225

GTX03225 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with alpha 1 Spectrin antibody [GT1313] (GTX03225) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-alpha 1 Spectrin antibody [GT1313] used in Western Blot (WB). GTX03225

GTX03225 WB Image

WB analysis of various samples using GTX03225 alpha 1 Spectrin antibody [GT1313].
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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Package List Price ($)
$ 439