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alpha 1 Spectrin antibody [SPTA1/1810]

Anti-alpha 1 Spectrin antibody [SPTA1/1810] used in IHC (Paraffin sections) (IHC-P). GTX17998
Anti-alpha 1 Spectrin antibody [SPTA1/1810] used in IHC (Paraffin sections) (IHC-P). GTX17998
Anti-alpha 1 Spectrin antibody [SPTA1/1810] used in Protein Array (Protein Array). GTX17998

Cat No. GTX17998

Host

Mouse

Clonality

Monoclonal

Clone Name

SPTA1/1810

Isotype

IgG2c

Application

IHC-P, ELISA, Protein Array

Reactivity

Human
Package
100 μg ($419)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P 1-2μg/ml for 30 minutes at RT
ELISA 2-4μg/ml (for coating)
Protein Array Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).

Not tested in other applications.

Calculated MW

280 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human SPTA fragment (around aa356-475) (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

EL2 , HPP , HS3 , SPH3 , SPTA , SPTA1 , spectrin alpha, erythrocytic 1 , alpha 1 Spectrin

Cellular Localization

Cytoplasm,Cytoskeleton,Cell cortex

Background

This gene encodes a member of a family of molecular scaffold proteins that link the plasma membrane to the actin cytoskeleton and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. The encoded protein is primarily composed of 22 spectrin repeats which are involved in dimer formation. It forms a component of the erythrocyte plasma membrane. Mutations in this gene result in a variety of hereditary red blood cell disorders, including elliptocytosis-2, pyropoikilocytosis, and spherocytosis, type 3. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-alpha 1 Spectrin antibody [SPTA1/1810] used in IHC (Paraffin sections) (IHC-P). GTX17998

GTX17998 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX17998 alpha 1 Spectrin antibody [SPTA1/1810].

Anti-alpha 1 Spectrin antibody [SPTA1/1810] used in IHC (Paraffin sections) (IHC-P). GTX17998

GTX17998 IHC-P Image

IHC-P analysis of human placenta tissue using GTX17998 alpha 1 Spectrin antibody [SPTA1/1810].

Anti-alpha 1 Spectrin antibody [SPTA1/1810] used in Protein Array (Protein Array). GTX17998

GTX17998 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using Spectrin, alpha 1 Mouse Monoclonal Antibody (SPTA1/1810). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf