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alpha 1 Spectrin antibody

Anti-alpha 1 Spectrin antibody used in IHC (Paraffin sections) (IHC-P). GTX64806
Anti-alpha 1 Spectrin antibody used in Western Blot (WB). GTX64806
Anti-alpha 1 Spectrin antibody used in IHC (Paraffin sections) (IHC-P). GTX64806
Anti-alpha 1 Spectrin antibody used in IHC (Paraffin sections) (IHC-P). GTX64806
Anti-alpha 1 Spectrin antibody used in IHC (Paraffin sections) (IHC-P). GTX64806
Anti-alpha 1 Spectrin antibody used in IHC (Paraffin sections) (IHC-P). GTX64806

Cat No. GTX64806

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

280 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 940-1160 of human SPTA1 (NP_003117.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

spectrin alpha, erythrocytic 1 , EL2 , HPP , HS3 , SPH3 , SPTA

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene encodes a member of a family of molecular scaffold proteins that link the plasma membrane to the actin cytoskeleton and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. The encoded protein is primarily composed of 22 spectrin repeats which are involved in dimer formation. It forms a component of the erythrocyte plasma membrane. Mutations in this gene result in a variety of hereditary red blood cell disorders, including elliptocytosis-2, pyropoikilocytosis, and spherocytosis, type 3. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-alpha 1 Spectrin antibody used in IHC (Paraffin sections) (IHC-P). GTX64806

GTX64806 IHC-P Image

IHC-P analysis of rat spleen tissue using GTX64806 alpha 1 Spectrin antibody.
Dilution : 1:100

Anti-alpha 1 Spectrin antibody used in Western Blot (WB). GTX64806

GTX64806 WB Image

WB analysis of various sample lysates using GTX64806 alpha 1 Spectrin antibody.
Dilution : 1:3000
Loading : 25μg per lane

Anti-alpha 1 Spectrin antibody used in IHC (Paraffin sections) (IHC-P). GTX64806

GTX64806 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX64806 alpha 1 Spectrin antibody.
Dilution : 1:100

Anti-alpha 1 Spectrin antibody used in IHC (Paraffin sections) (IHC-P). GTX64806

GTX64806 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX64806 alpha 1 Spectrin antibody.
Dilution : 1:100

Anti-alpha 1 Spectrin antibody used in IHC (Paraffin sections) (IHC-P). GTX64806

GTX64806 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX64806 alpha 1 Spectrin antibody.
Blue : DAPI
Dilution : 1:100

Anti-alpha 1 Spectrin antibody used in IHC (Paraffin sections) (IHC-P). GTX64806

GTX64806 IHC-P Image

IHC-P analysis of rat bone marrow tissue using GTX64806 alpha 1 Spectrin antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf