alpha 1a Adrenergic Receptor antibody

Cat. No. GTX100190

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat, Cat
Package	100 μl ($399), 25 μl ($169)

See all alpha 1a Adrenergic Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

Raji , Jurkat , Raji , NCI-H929

Predict Reactivity

Rabbit, Bovine, Dog, Guinea pig, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human alpha 1a Adrenergic Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1080925

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adrenoceptor alpha 1A , ADRA1C , ADRA1L1 , ALPHA1AAR

Cellular Localization

Cell membrane

Background

Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1A-adrenergic receptor. Alternative splicing of this gene generates four transcript variants, which encode four different isoforms with distinct C-termini but having similar ligand binding properties. [provided by RefSeq]

Database

Gene ID: 148 ADRA1A
UniProt: P35348 ADRA1A

Research Area

DATA IMAGES

GTX100190 ICC/IF Image

alpha 1a Adrenergic Receptor antibody detects alpha 1a Adrenergic Receptor protein at cytoplasm by immunofluorescent analysis.
Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha 1a Adrenergic Receptor stained by alpha 1a Adrenergic Receptor antibody (GTX100190) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

GTX100190 IHC-P Image

alpha 1a Adrenergic Receptor antibody detects alpha 1a Adrenergic Receptor protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
alpha 1a Adrenergic Receptor antibody (GTX100190) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100190 WB Image

Sample(30 μg of whole cell lysate)
A:Raji (GTX27908)
10% SDS PAGE
GTX100190 diluted at 1:1000

GTX100190 IHC-P Image

alpha 1a Adrenergic Receptor antibody detects alpha 1a Adrenergic Receptor protein at cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
alpha 1a Adrenergic Receptor antibody (GTX100190) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100190 WB Image

alpha 1a Adrenergic Receptor antibody detects alpha 1a Adrenergic Receptor protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
10 % SDS-PAGE
alpha 1a Adrenergic Receptor antibody (GTX100190) dilution: 1:1000

GTX100190 ICC/IF Image

Immunofluorescence analysis of methanol-fixed Hep3B, using alpha 1a Adrenergic Receptor(GTX100190) antibody at 1:500 dilution.

GTX100190 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human heart, using alpha 1a Adrenergic Receptor(GTX100190) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Choi BR et al. Drug Des Devel Ther 2018; 12 : 1855-1863 Additive effect of oral LDD175 to * and * in a benign prostate hyperplasia rat model.

Application : WB

Reactivity : Rat

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REVIEW

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Submit a Review

Application Reference

Choi BR et al. Drug Des Devel Ther 2018; 12:1855-1863 Additive effect of oral LDD175 to * and * in a benign prostate hyperplasia rat model.

Application : WB

Reactivity : Rat

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

Document
Unmodified.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty

alpha 1a Adrenergic Receptor antibody

Cat. No. GTX100190

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Positive Control

Predict Reactivity

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX100190 ICC/IF Image

GTX100190 IHC-P Image

GTX100190 WB Image

GTX100190 IHC-P Image

GTX100190 WB Image

GTX100190 ICC/IF Image

GTX100190 IHC-P Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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