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alpha 1a Adrenergic Receptor antibody

Anti-alpha 1a Adrenergic Receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX100190
Anti-alpha 1a Adrenergic Receptor antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100190
Anti-alpha 1a Adrenergic Receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX100190
Anti-alpha 1a Adrenergic Receptor antibody used in Western Blot (WB). GTX100190
Anti-alpha 1a Adrenergic Receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX100190
Anti-alpha 1a Adrenergic Receptor antibody used in Western Blot (WB). GTX100190

Cat No. GTX100190

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

Jurkat , Raji , NCI-H929

Predict Reactivity

Rabbit, Bovine, Dog, Guinea pig, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human alpha 1a Adrenergic Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

adrenoceptor alpha 1A , ADRA1C , ADRA1L1 , ALPHA1AAR

Cellular Localization

Cell membrane

Background

Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1A-adrenergic receptor. Alternative splicing of this gene generates four transcript variants, which encode four different isoforms with distinct C-termini but having similar ligand binding properties. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-alpha 1a Adrenergic Receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX100190

GTX100190 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human heart, using alpha 1a Adrenergic Receptor(GTX100190) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha 1a Adrenergic Receptor antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100190

GTX100190 ICC/IF Image

Immunofluorescence analysis of methanol-fixed Hep3B, using alpha 1a Adrenergic Receptor(GTX100190) antibody at 1:500 dilution.

Anti-alpha 1a Adrenergic Receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX100190

GTX100190 IHC-P Image

alpha 1a Adrenergic Receptor antibody detects alpha 1a Adrenergic Receptor protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
alpha 1a Adrenergic Receptor antibody (GTX100190) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha 1a Adrenergic Receptor antibody used in Western Blot (WB). GTX100190

GTX100190 WB Image

Sample(30 μg of whole cell lysate)
A:Raji (GTX27908)
10% SDS PAGE
GTX100190 diluted at 1:1000

Anti-alpha 1a Adrenergic Receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX100190

GTX100190 IHC-P Image

alpha 1a Adrenergic Receptor antibody detects alpha 1a Adrenergic Receptor protein at cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
alpha 1a Adrenergic Receptor antibody (GTX100190) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha 1a Adrenergic Receptor antibody used in Western Blot (WB). GTX100190

GTX100190 WB Image

alpha 1a Adrenergic Receptor antibody detects alpha 1a Adrenergic Receptor protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
10 % SDS-PAGE
alpha 1a Adrenergic Receptor antibody (GTX100190) dilution: 1:1000

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169