APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Raji , Jurkat , Raji , NCI-H929
Predict Reactivity
Rabbit, Bovine, Dog, Guinea pig, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide encompassing a sequence within the Intracellular domain of human alpha 1a Adrenergic Receptor. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1080925
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
adrenoceptor alpha 1A , ADRA1C , ADRA1L1 , ALPHA1AAR
Cellular Localization
Cell membrane
Background
Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1A-adrenergic receptor. Alternative splicing of this gene generates four transcript variants, which encode four different isoforms with distinct C-termini but having similar ligand binding properties. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX100190 IHC-P Image
alpha 1a Adrenergic Receptor antibody detects alpha 1a Adrenergic Receptor protein at cytosol on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. alpha 1a Adrenergic Receptor antibody (GTX100190) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX100190 WB Image
Sample(30 μg of whole cell lysate) A:Raji (GTX27908) 10% SDS PAGE GTX100190 diluted at 1:1000
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GTX100190 IHC-P Image
alpha 1a Adrenergic Receptor antibody detects alpha 1a Adrenergic Receptor protein at cytosol on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. alpha 1a Adrenergic Receptor antibody (GTX100190) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX100190 WB Image
alpha 1a Adrenergic Receptor antibody detects alpha 1a Adrenergic Receptor protein by western blot analysis. A. 30 μg Jurkat whole cell lysate/extract B. 30 μg Raji whole cell lysate/extract C. 30 μg NCI-H929 whole cell lysate/extract 10 % SDS-PAGE alpha 1a Adrenergic Receptor antibody (GTX100190) dilution: 1:1000
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GTX100190 ICC/IF Image
Immunofluorescence analysis of methanol-fixed Hep3B, using alpha 1a Adrenergic Receptor(GTX100190) antibody at 1:500 dilution.
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GTX100190 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human heart, using alpha 1a Adrenergic Receptor(GTX100190) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX100190 ICC/IF Image
alpha 1a Adrenergic Receptor antibody detects alpha 1a Adrenergic Receptor protein at cytoplasm by immunofluorescent analysis.Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: alpha 1a Adrenergic Receptor stained by alpha 1a Adrenergic Receptor antibody (GTX100190) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 μm.
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REFERENCE
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