alpha 1b Adrenergic Receptor antibody [HL4479]

Unboiled various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha 1b Adrenergic Receptor antibody [HL4479] (GTX644046) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data are based on NCBI database.

Unboiled various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha 1b Adrenergic Receptor antibody [HL4479] (GTX644046) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Unboiled U2OS whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha 1b Adrenergic Receptor antibody [HL4479] (GTX644046) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (WCE: whole cell extract; ME: membrane extract)

Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha 1b Adrenergic Receptor antibody [HL4479] (GTX644046) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.