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alpha 2 Macroglobulin antibody

Anti-alpha 2 Macroglobulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32434
Anti-alpha 2 Macroglobulin antibody used in Western Blot (WB). GTX32434

Cat. No. GTX32434

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all alpha 2 Macroglobulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

163 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1155-1474 of human A2M (NP_000005.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887656

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

alpha-2-macroglobulin , A2MD , CPAMD5 , FWP007 , S863-7

Cellular Localization

Secreted

Background

The protein encoded by this gene is a protease inhibitor and cytokine transporter. It uses a bait-and-trap mechanism to inhibit a broad spectrum of proteases, including trypsin, thrombin and collagenase. It can also inhibit inflammatory cytokines, and it thus disrupts inflammatory cascades. Mutations in this gene are a cause of alpha-2-macroglobulin deficiency. This gene is implicated in Alzheimer's disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. A related pseudogene, which is also located on the p arm of chromosome 12, has been identified. [provided by RefSeq, Nov 2016]

Database

  • Gene ID: 2 A2M
  • UniProt: P01023 A2M

Research Area

DATA IMAGES

Anti-alpha 2 Macroglobulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32434

GTX32434 ICC/IF Image

ICC/IF analysis of 293T cells using GTX32434 alpha 2 Macroglobulin antibody.
Blue : DAPI
Dilution : 1:100

Anti-alpha 2 Macroglobulin antibody used in Western Blot (WB). GTX32434

GTX32434 WB Image

WB analysis of various sample lysates using GTX32434 alpha 2 Macroglobulin antibody.
Dilution : 1:500
Loading : 25μg per lane

REFERENCE

Application Reference

Hsu WH et al. PLoS One 2019; 14 (1) : e0209184 Combined proteomic and metabolomic analyses of cerebrospinal fluid from mice with ischemic stroke reveals the effects of a Buyang Huanwu decoction in neurodegenerative disease.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399