alpha 2a Adrenergic Receptor antibody [HL3723]

Unboiled non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha 2a Adrenergic Receptor antibody [HL3723] (GTX641913) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) unboiled mouse tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha 2a Adrenergic Receptor antibody [HL3723] (GTX641913) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Boiled and unboiled LNCap whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha 2a Adrenergic Receptor antibody [HL3723] (GTX641913) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)