Unboiled non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha 2a Adrenergic Receptor antibody [HL3725] (GTX641915) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

alpha 2a Adrenergic Receptor antibody [HL3725] detects alpha 2a Adrenergic Receptor protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
alpha 2a Adrenergic Receptor stained by alpha 2a Adrenergic Receptor antibody [HL3725] (GTX641915) diluted at 1:100.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Unboiled LNCap whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha 2a Adrenergic Receptor antibody [HL3725] (GTX641915) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (WCE: whole cell extract; ME: membrane extract)