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alpha A Crystallin antibody [GT1276]

Recombinant antibody
Anti-alpha A Crystallin antibody [GT1276] used in Western Blot (WB). GTX03188
Anti-alpha A Crystallin antibody [GT1276] used in Western Blot (WB). GTX03188
Anti-alpha A Crystallin antibody [GT1276] used in Western Blot (WB). GTX03188
Anti-alpha A Crystallin antibody [GT1276] used in IHC (Paraffin sections) (IHC-P). GTX03188
Anti-alpha A Crystallin antibody [GT1276] used in IHC (Paraffin sections) (IHC-P). GTX03188

Cat. No. GTX03188

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1276

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Mouse, Rat
Package
100 μl ($439)
See all alpha A Crystallin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human CRYAA.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

crystallin alpha A , CRYA1 , CTRCT9 , HSPB4

Cellular Localization

Cytoplasm , Nucleus

Background

Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC). [provided by RefSeq, Jan 2014]

Database

Research Area

DATA IMAGES

Anti-alpha A Crystallin antibody [GT1276] used in Western Blot (WB). GTX03188

GTX03188 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with alpha A Crystallin antibody [GT1276] (GTX03188) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha A Crystallin antibody [GT1276] used in Western Blot (WB). GTX03188

GTX03188 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with alpha A Crystallin antibody [GT1276] (GTX03188) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha A Crystallin antibody [GT1276] used in Western Blot (WB). GTX03188

GTX03188 WB Image

WB analysis of various samples using GTX03188 alpha A Crystallin antibody [GT1276].
Dilution : 1:1000
Loading : 25μg per lane

Anti-alpha A Crystallin antibody [GT1276] used in IHC (Paraffin sections) (IHC-P). GTX03188

GTX03188 IHC-P Image

IHC-P analysis of rat eye tissue section using GTX03188 alpha A Crystallin antibody [GT1276].
Blue : DAPI for nuclear staining
Dilution : 1:100

Anti-alpha A Crystallin antibody [GT1276] used in IHC (Paraffin sections) (IHC-P). GTX03188

GTX03188 IHC-P Image

IHC-P analysis of mouse eye tissue section using GTX03188 alpha A Crystallin antibody [GT1276].
Blue : DAPI for nuclear staining
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 439