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alpha A Crystallin antibody [c9F2]

Anti-alpha A Crystallin antibody [c9F2] used in Western Blot (WB). GTX50077

Cat. No. GTX50077

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 c9F2

Isotype

 IgG1

Application

 WB, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all alpha A Crystallin products

APPLICATION

Application Note

We recommend the following starting dilutions:
Western blot analysis: 1:500 ~ 1:2000.

Optimal working concentrations should be determined experimentally by the end user.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human alpha A Crystallin (1-173aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

crystallin alpha A , CRYA1 , CTRCT9 , HSPB4

Cellular Localization

Cytoplasm , Nucleus

Background

Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC). [provided by RefSeq, Jan 2014]

Database

Research Area

DATA IMAGES

Anti-alpha A Crystallin antibody [c9F2] used in Western Blot (WB). GTX50077

GTX50077 WB Image

Mouse eye extracts and recombinant proteins (Crystallin-alpha A and B) were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-Crystallin alpha A (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody con

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399