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alpha Actinin 1 antibody [0.T.02]

Anti-alpha Actinin 1 antibody [0.T.02] used in Western Blot (WB). GTX18061

Cat No. GTX18061

Host

Mouse

Clonality

Monoclonal

Clone Name

0.T.02

Isotype

IgG1

Application

WB, ICC/IF, IHC-Fr

Reactivity

Human, Mouse, Rat, Xenopus
Package
100 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-2μg/ml
ICC/IF 15μg/ml (fixed with methanol)
IHC-Fr 15μg/ml
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

Product Note

Recognizes human alpha-Actinin 1 isoform (non-muscle) at 100kD.

PROPERTIES

Form

Liquid

Buffer

0.02M PBS, 0.25M NaCl, 0.1% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human alpha-Actinin

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_423052

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ACTN1 , BDPLT15 , actinin alpha 1 , alpha Actinin 1

Cellular Localization

Cytoplasm,Cytoskeleton,Myofibril,Sarcomere,Z line,Cell membrane,Cell junction,Cell projection,Ruffle

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

  • Gene ID: 87 ACTN1
  • UniProt: P12814 ACTN1

Research Area

DATA IMAGES

Anti-alpha Actinin 1 antibody [0.T.02] used in Western Blot (WB). GTX18061

GTX18061 WB Image

WB analysis of A431 cell lysate using GTX18061 alpha Actinin 1 antibody [0.T.02].

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379