alpha Actinin 1 antibody [AT1D10]

Cat. No. GTX57633

Host	Mouse
Clonality	Monoclonal
Clone Name	AT1D10
Isotype	IgG2a
Application	WB, ICC/IF, FACS
Reactivity	Human, Mouse
Package	100 μl ($399)

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Datasheet
Document

View product citations for antibody GTX57633 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	Assay dependent
FACS	Assay dependent

Not tested in other applications.

Calculated MW

103 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT1D10 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ACTN3 protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actinin alpha 1 , ACTN1 , BDPLT15 , alpha Actinin 1

Cellular Localization

Cytoplasm,Cytoskeleton,Myofibril,Sarcomere,Z line,Cell membrane,Cell junction,Cell projection,Ruffle

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Gene ID: 87 ACTN1
UniProt: P12814 ACTN1

Research Area

DATA IMAGES

GTX57633 WB Image

WB analysis of various samples using GTX57633 alpha Actinin 1 antibody.
Lane 1 : PC-3 whole cell lysate
Lane 2 : A549 whole cell lysate
Loading : 40 μg
Dilution : 1:1000

GTX57633 ICC/IF Image

ICC/IF analysis of Balb/3T3 cells using GTX57633 alpha Actinin 1 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

GTX57633 FACS Image

FACS analysis of HeLa cells using GTX57633 alpha Actinin 1 antibody.
Cell Number: 1 x 10⁶ cells
Red: Primary antibody
Antibody amount: 2-5 μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty