GeneTex
  • Country / Location Selection

United States (US)

alpha Actinin 1 antibody [AT1D10]

Anti-alpha Actinin 1 antibody [AT1D10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57633
Anti-alpha Actinin 1 antibody [AT1D10] used in Western Blot (WB). GTX57633
Anti-alpha Actinin 1 antibody [AT1D10] used in Flow cytometry (FACS). GTX57633

Cat No. GTX57633

Host

Mouse

Clonality

Monoclonal

Clone Name

AT1D10

Isotype

IgG2a

Application

WB, ICC/IF, FACS

Reactivity

Human, Mouse
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (pH 7.4), 0.02% sodium azide, 10% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT1D10 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ACTN3 protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

actinin alpha 1 , ACTN1 , BDPLT15 , alpha Actinin 1

Cellular Localization

Cytoplasm,Cytoskeleton,Myofibril,Sarcomere,Z line,Cell membrane,Cell junction,Cell projection,Ruffle

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

  • Gene ID: 87 ACTN1
  • UniProt: P12814 ACTN1

Research Area

DATA IMAGES

Anti-alpha Actinin 1 antibody [AT1D10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57633

GTX57633 ICC/IF Image

ICC/IF analysis of Balb/3T3 cells using GTX57633 alpha Actinin 1 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

Anti-alpha Actinin 1 antibody [AT1D10] used in Western Blot (WB). GTX57633

GTX57633 WB Image

WB analysis of various samples using GTX57633 alpha Actinin 1 antibody.
Lane 1 : PC-3 whole cell lysate
Lane 2 : A549 whole cell lysate
Loading : 40 μg
Dilution : 1:1000

Anti-alpha Actinin 1 antibody [AT1D10] used in Flow cytometry (FACS). GTX57633

GTX57633 FACS Image

FACS analysis of HeLa cells using GTX57633 alpha Actinin 1 antibody.
Cell Number: 1 x 10⁶ cells
Red: Primary antibody
Antibody amount: 2-5 μg

REFERENCE

There are currently no references for alpha Actinin 1 antibody [AT1D10] (GTX57633). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for alpha Actinin 1 antibody [AT1D10] (GTX57633). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339