APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
NIH-3T3 , 293T , A431 , HeLa , HepG2 , PC-12 , Rat2 , rat brain
Predict Reactivity
Bovine, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.09 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human alpha Actinin 1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1949576
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
actinin alpha 1 , BDPLT15
Cellular Localization
Cytoplasm , cytoskeleton , myofibril , sarcomere , Z-disk
Background
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
|
GTX103240 WB Image
Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 1 antibody [N2N3] (GTX103240) diluted at 1:1000.
|
|
GTX103240 IHC-Wm Image
alpha Actinin 1 antibody [N2N3] detects alpha Actinin 1 protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. alpha Actinin 1 antibody [N2N3] (GTX103240) dilution: 1:50.
|
|
GTX103240 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 1 antibody [N2N3] (GTX103240) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX103240 WB Image
Sample (30 μg of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE GTX103240 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX103240 WB Image
alpha Actinin 1 antibody detects alpha Actinin 1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 1 antibody (GTX103240) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX103240 WB Image
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 1 antibody [N2N3] (GTX103240) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX103240 IHC-P Image
Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using Alpha-actinin 1(GTX103240) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
REFERENCE
There are currently no references for alpha Actinin 1 antibody [N2N3] (GTX103240). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for alpha Actinin 1 antibody [N2N3] (GTX103240). Be the first to share your experience with this product.