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alpha Actinin 1 antibody [N2N3]

Anti-alpha Actinin 1 antibody [N2N3] used in Western Blot (WB). GTX103240
Anti-alpha Actinin 1 antibody [N2N3] used in IHC (Whole mount) (IHC-Wm). GTX103240
Anti-alpha Actinin 1 antibody [N2N3] used in Western Blot (WB). GTX103240
Anti-alpha Actinin 1 antibody [N2N3] used in Western Blot (WB). GTX103240
Anti-alpha Actinin 1 antibody [N2N3] used in Western Blot (WB). GTX103240
Anti-alpha Actinin 1 antibody [N2N3] used in Western Blot (WB). GTX103240
Anti-alpha Actinin 1 antibody [N2N3] used in IHC (Paraffin sections) (IHC-P). GTX103240

Cat. No. GTX103240

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Wm

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all alpha Actinin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

Positive Control

NIH-3T3 , 293T , A431 , HeLa , HepG2 , PC-12 , Rat2 , rat brain

Predict Reactivity

Bovine, Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.09 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Actinin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949576

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actinin alpha 1 , BDPLT15

Cellular Localization

Cytoplasm , cytoskeleton , myofibril , sarcomere , Z-disk

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

  • Gene ID: 87 ACTN1
  • UniProt: P12814 ACTN1

Research Area

DATA IMAGES

Anti-alpha Actinin 1 antibody [N2N3] used in Western Blot (WB). GTX103240

GTX103240 WB Image

Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 1 antibody [N2N3] (GTX103240) diluted at 1:1000.

Anti-alpha Actinin 1 antibody [N2N3] used in IHC (Whole mount) (IHC-Wm). GTX103240

GTX103240 IHC-Wm Image

alpha Actinin 1 antibody [N2N3] detects alpha Actinin 1 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
alpha Actinin 1 antibody [N2N3] (GTX103240) dilution: 1:50.
Anti-alpha Actinin 1 antibody [N2N3] used in Western Blot (WB). GTX103240

GTX103240 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 1 antibody [N2N3] (GTX103240) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 1 antibody [N2N3] used in Western Blot (WB). GTX103240

GTX103240 WB Image

Sample (30 μg of whole cell lysate)
A:NIH-3T3
7.5% SDS PAGE
GTX103240 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 1 antibody [N2N3] used in Western Blot (WB). GTX103240

GTX103240 WB Image

alpha Actinin 1 antibody detects alpha Actinin 1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 1 antibody (GTX103240) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 1 antibody [N2N3] used in Western Blot (WB). GTX103240

GTX103240 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 1 antibody [N2N3] (GTX103240) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 1 antibody [N2N3] used in IHC (Paraffin sections) (IHC-P). GTX103240

GTX103240 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using Alpha-actinin 1(GTX103240) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169