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alpha Actinin 2 antibody [GT1253]

Anti-alpha Actinin 2 antibody [GT1253] used in Western Blot (WB). GTX632361
Anti-alpha Actinin 2 antibody [GT1253] used in Western Blot (WB). GTX632361
Anti-alpha Actinin 2 antibody [GT1253] used in IHC (Paraffin sections) (IHC-P). GTX632361

Cat. No. GTX632361

Host

Mouse

Clonality

Monoclonal

Clone Name

GT1253

Isotype

IgG1

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

104 kDa. ( Note )

Positive Control

HepG2

Product Note

Based on sequence homology, this antibody may cross react with ACTN1 protein.

Predict Reactivity

Rat, Drosophila, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Actinin 2. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888310

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

actinin alpha 2 , CMD1AA , CMH23

Cellular Localization

Cytoplasm , myofibril , sarcomere , Z-disk

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. [provided by RefSeq]

Database

  • Gene ID: 88 ACTN2
  • UniProt: P35609 ACTN2

Research Area

DATA IMAGES

Anti-alpha Actinin 2 antibody [GT1253] used in Western Blot (WB). GTX632361

GTX632361 WB Image

Mouse tissue extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [GT1253] (GTX632361) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-alpha Actinin 2 antibody [GT1253] used in Western Blot (WB). GTX632361

GTX632361 WB Image

alpha Actinin 2 antibody detects alpha Actinin 2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody (GTX632361) at a dilution of 1:1000.

Anti-alpha Actinin 2 antibody [GT1253] used in IHC (Paraffin sections) (IHC-P). GTX632361

GTX632361 IHC-P Image

alpha Actinin 2 antibody [GT1253] detects alpha Actinin 2 protein at cytoplasm in mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
alpha Actinin 2 antibody [GT1253] (GTX632361) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations