alpha Actinin 2 antibody [N1N3]

Name: alpha Actinin 2 antibody [N1N3]
Brand: GeneTex
SKU: GTX103219
Price: 399.00 USD
Rating: 5 (2 reviews)

Anti-alpha Actinin 2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103219

Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219

Anti-alpha Actinin 2 antibody [N1N3] used in Western Blot (WB). GTX103219

Cat. No. GTX103219

Reference 12

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC
Reactivity	Human, Mouse, Rat, Zebrafish, Pig
Package	100 μl ($399), 25 μl ($169)

See all alpha Actinin 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC	Assay dependent

Not tested in other applications.

Calculated MW

104 kDa. ( Note )

Product Note

Based on sequence homology, this antibody may cross-react with other Actinin family members.

Predict Reactivity

Drosophila, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Actinin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949578

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

actinin alpha 2 , CMD1AA , CMH23

Cellular Localization

Cytoplasm , myofibril , sarcomere , Z-disk

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. [provided by RefSeq]

Database

Gene ID: 88 ACTN2
UniProt: P35609 ACTN2

Research Area

DATA IMAGES

GTX103219 ICC/IF Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX103219 ICC/IF Image

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm in rat muscle by immunohistochemical analysis.
Sample: Paraffin-embedded rat muscle.
alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX103219 ICC/IF Image

GTX103219 IHC-P Image

GTX103219 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103219 WB Image

Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103219 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX103219 WB Image

Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Boimpoundi Eunice Flavie Ouali et al. Biochem Biophys Rep 2021; 26 : 101019 Beta-agonist drugs modulate the proliferation and differentiation of skeletal muscle cells in vitro.

Application : WB

Reactivity : Mouse

Cowan J et al. Sci Rep 2021; 11 (1) : 6293 Regulation of cardiomyocyte DNA damage and cell death by the type 2A protein phosphatase regulatory protein alpha4.

Weber B et al. Sci Rep 2021; 11 (1) : 8503 Early myocardial damage (EMD) and valvular dysfunction after femur fracture in pigs.

Application : IHC-P

Reactivity : Pig

Masita Silviana N et al. Turk J Pharm Sci 2021; 18 (1) : 28-33 Masticatory Functional Load Increases the mRNA Expression Levels of ACTN2 and ACTN3 and the Protein Expression of α-Actinin-2 in Rat Masseter Muscle.

Application : WB

Reactivity : Rat

Li H et al. Cells 2019; 8 (9) PFN2a Suppresses C2C12 Myogenic Development by Inhibiting Proliferation and Promoting Apoptosis via the p53 Pathway.

Application : WB

Reactivity : Human

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