GeneTex
United States (US)

alpha Actinin 2 antibody [N1N3]

GTX103219_42655_20161110_WB_19011506_178.jpg
GTX103219_42656_20181025_IHC-P_M_19011519_160.jpg
GTX103219_42656_20181025_IHC-P_R_19011519_662.jpg
GTX103219_41241_WB_M_19011506_899.jpg
GTX103219_42656_20170315_IFA_19011506_504.jpg
GTX103219_39974_IHC_19011506_942.jpg
GTX103219_42655_20170426_IHC-P_M_19011506_237.jpg
GTX103219_WB_M_19011506_114.jpg
GTX103219_41794_20150903_WB_R_heart_19011506_705.jpg
GTX103219_42655_20170426_IHC-P_R_19011506_940.jpg

Cat No. GTX103219

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat, Pig
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

104 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Neuro2A , GL261 , C8D30 , NIH-3T3 , Rat heart

Predict Reactivity

Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.390 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Actinin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Actinin Alpha 2,Cmd1Aa,Cmh23,Actn2

Cellular Localization

Cytoplasm , myofibril , sarcomere , Z-disk

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103219_42655_20161110_WB_19011506_178.jpg

GTX103219 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103219_42656_20181025_IHC-P_M_19011519_160.jpg

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX103219_42656_20181025_IHC-P_R_19011519_662.jpg

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX103219_41241_WB_M_19011506_899.jpg

GTX103219 WB Image

Alpha Actinin 2 antibody [N1N3] detects ACTN2 protein by western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
7.5% SDS-PAGE
alpha Actinin 2 antibody [N1N3] (GTX103219) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103219_42656_20170315_IFA_19011506_504.jpg

GTX103219 ICC/IF Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: alpha Actinin 2 protein stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX103219_39974_IHC_19011506_942.jpg

GTX103219 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human heart, using alpha Actinin 2(GTX103219) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103219_42655_20170426_IHC-P_M_19011506_237.jpg

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm in mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX103219_WB_M_19011506_114.jpg

GTX103219 WB Image

Sample (30 µg of whole cell lysate)
A:NIH-3T3
7.5% SDS PAGE
GTX103219 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103219_41794_20150903_WB_R_heart_19011506_705.jpg

GTX103219 WB Image

alpha Actinin 2 antibody detects alpha Actinin 2 protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody (GTX103219) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103219_42655_20170426_IHC-P_R_19011506_940.jpg

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm in rat muscle by immunohistochemical analysis.
Sample: Paraffin-embedded rat muscle.
alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169