APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:10000 |
1:100-1:1000 |
1:100-1:1000 |
Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
Based on sequence homology, this antibody may cross-react with other Actinin family members.
Predict Reactivity
Drosophila, Bovine, Chicken, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS pH7, 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.18 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human alpha Actinin 2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1949578
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
actinin alpha 2 , CMD1AA , CMH23
Cellular Localization
Cytoplasm , myofibril , sarcomere , Z-disk
Background
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX103219 ICC/IF Image
alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX103219 IHC-P Image
alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat heart.alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX103219 IHC-P Image
alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse heart.alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX103219 IHC-P Image
alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse muscle.alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX103219 ICC/IF Image
alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.Blue: Hoechst 33342 staining.
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GTX103219 IHC-P Image
alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm in rat muscle by immunohistochemical analysis. Sample: Paraffin-embedded rat muscle. alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX103219 IHC-P Image
alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse heart.alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX103219 ICC/IF Image
alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX103219 IHC-P Image
alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse muscle.alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX103219 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX103219 WB Image
Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX103219 WB Image
Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX103219 IHC-P Image
alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse muscle.alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX103219 IHC-P Image
alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse heart.alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX103219 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX103219 WB Image
Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX103219 IHC-P Image
alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse heart.alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX103219 IHC-P Image
alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat heart.alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
REVIEW
alpha Actinin 2 antibody [N1N3]
Cat. No. GTX103219
Rating
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( Average 5 based on 2 users reviews)
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Application
Western Blot(WB)
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( Average 5 based on 2 users reviews)
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Date :
Anonymous submitted on 14-Dec-2021
Application Tested :
WB
Sample Species :
Rat
Sample :
rat skeletal muscle tissue
Amount used :
2.5-10μg
Blocking :
3% skim milk, 25°C, 1Hr
Primary Antibody :
1:2000, 4°C, 12Hr
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PeerJ in 2018.
PMID: 30065891