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alpha Actinin 2 antibody [N1N3]

Anti-alpha Actinin 2 antibody [N1N3] used in Western Blot (WB). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in Western Blot (WB). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in Western Blot (WB). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in Western Blot (WB). GTX103219

Cat No. GTX103219

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC

Reactivity

Human, Mouse, Rat, Pig
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

104 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Neuro2A , GL261 , C8D30 , NIH-3T3 , Rat heart

Predict Reactivity

Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS,20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Actinin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949578

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

actinin alpha 2 , CMD1AA , CMH23

Cellular Localization

Cytoplasm , myofibril , sarcomere , Z-disk

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. [provided by RefSeq]

Database

  • Gene ID: 88 ACTN2
  • UniProt: P35609 ACTN2

Research Area

DATA IMAGES

Anti-alpha Actinin 2 antibody [N1N3] used in Western Blot (WB). GTX103219

GTX103219 WB Image

Alpha Actinin 2 antibody [N1N3] detects ACTN2 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
7.5% SDS-PAGE
alpha Actinin 2 antibody [N1N3] (GTX103219) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 2 antibody [N1N3] used in Western Blot (WB). GTX103219

GTX103219 WB Image

Sample (30 μg of whole cell lysate)
A:NIH-3T3
7.5% SDS PAGE
GTX103219 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Actinin 2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103219

GTX103219 ICC/IF Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-alpha Actinin 2 antibody [N1N3] used in Western Blot (WB). GTX103219

GTX103219 WB Image

alpha Actinin 2 antibody detects alpha Actinin 2 protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody (GTX103219) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm in rat muscle by immunohistochemical analysis.
Sample: Paraffin-embedded rat muscle.
alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Actinin 2 antibody [N1N3] used in Western Blot (WB). GTX103219

GTX103219 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

alpha Actinin 2 antibody [N1N3] Cat No. GTX103219

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PeerJ in 2018.PMID: 30065891
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf