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alpha Actinin 2 antibody [N1N3]

Anti-alpha Actinin 2 antibody [N1N3] used in Western Blot (WB). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in Western Blot (WB). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in Western Blot (WB). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in Western Blot (WB). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in Western Blot (WB). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in Western Blot (WB). GTX103219

Cat. No. GTX103219

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC

Reactivity

Human, Mouse, Rat, Zebrafish, Pig
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

104 kDa. ( Note )

Product Note

Based on sequence homology, this antibody may cross-react with other Actinin family members.

Predict Reactivity

Drosophila, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Actinin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949578

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actinin alpha 2 , CMD1AA , CMH23

Cellular Localization

Cytoplasm , myofibril , sarcomere , Z-disk

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. [provided by RefSeq]

Database

  • Gene ID: 88 ACTN2
  • UniProt: P35609 ACTN2

Research Area

DATA IMAGES

Anti-alpha Actinin 2 antibody [N1N3] used in Western Blot (WB). GTX103219

GTX103219 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 2 antibody [N1N3] used in Western Blot (WB). GTX103219

GTX103219 WB Image

Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Actinin 2 antibody [N1N3] used in Western Blot (WB). GTX103219

GTX103219 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 2 antibody [N1N3] used in Western Blot (WB). GTX103219

GTX103219 WB Image

Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103219

GTX103219 ICC/IF Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Actinin 2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103219

GTX103219 ICC/IF Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm in rat muscle by immunohistochemical analysis.
Sample: Paraffin-embedded rat muscle.
alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Actinin 2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103219

GTX103219 ICC/IF Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Actinin 2 antibody [N1N3] used in Western Blot (WB). GTX103219

GTX103219 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 2 antibody [N1N3] used in Western Blot (WB). GTX103219

GTX103219 WB Image

The data was published in the journal PeerJ in 2018.PMID: 30065891

REFERENCE

REVIEW

alpha Actinin 2 antibody [N1N3] Cat. No. GTX103219

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 14-Dec-2021
Score :
Application Tested : WB
Sample Species : Rat
Sample : rat skeletal muscle tissue
Amount used : 2.5-10μg
Blocking : 3% skim milk, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 12Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169