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alpha Actinin 2 antibody [N1N3]

Anti-alpha Actinin 2 antibody [N1N3] used in Western Blot (WB). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in Western Blot (WB). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in Western Blot (WB). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in Western Blot (WB). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in Western Blot (WB). GTX103219
Anti-alpha Actinin 2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103219

Cat. No. GTX103219

Reference 13
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC

Reactivity

 Human, Mouse, Rat, Zebrafish, Pig
Package
100 μl ($399),
25 μl ($169)
See all alpha Actinin 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

104 kDa. ( Note )

Product Note

Based on sequence homology, this antibody may cross-react with other Actinin family members.

Predict Reactivity

Drosophila, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Actinin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949578

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actinin alpha 2 , CMD1AA , CMH23

Cellular Localization

Cytoplasm , myofibril , sarcomere , Z-disk

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. [provided by RefSeq]

Database

  • Gene ID: 88 ACTN2
  • UniProt: P35609 ACTN2

Research Area

DATA IMAGES

Anti-alpha Actinin 2 antibody [N1N3] used in Western Blot (WB). GTX103219

GTX103219 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 2 antibody [N1N3] used in Western Blot (WB). GTX103219

GTX103219 WB Image

Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Actinin 2 antibody [N1N3] used in Western Blot (WB). GTX103219

GTX103219 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Actinin 2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103219

GTX103219 ICC/IF Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Actinin 2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103219

GTX103219 ICC/IF Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm in rat muscle by immunohistochemical analysis.
Sample: Paraffin-embedded rat muscle.
alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Actinin 2 antibody [N1N3] used in Western Blot (WB). GTX103219

GTX103219 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103219

GTX103219 IHC-P Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Actinin 2 antibody [N1N3] used in Western Blot (WB). GTX103219

GTX103219 WB Image

Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103219

GTX103219 ICC/IF Image

alpha Actinin 2 antibody [N1N3] detects alpha Actinin 2 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Rodriguez Garcia M et al. Int J Mol Sci 2023; 24 (19) Disruption of Z-Disc Function Promotes Mechanical Dysfunction in Human Myocardium: Evidence for a Dual Myofilament Modulatory Role by Alpha-Actinin 2.
Boimpoundi Eunice Flavie Ouali et al. Biochem Biophys Rep 2021; 26 : 101019 Beta-agonist drugs modulate the proliferation and differentiation of skeletal muscle cells in vitro.
Application : WB
Reactivity : Mouse
Cowan J et al. Sci Rep 2021; 11 (1) : 6293 Regulation of cardiomyocyte DNA damage and cell death by the type 2A protein phosphatase regulatory protein alpha4.
Weber B et al. Sci Rep 2021; 11 (1) : 8503 Early myocardial damage (EMD) and valvular dysfunction after femur fracture in pigs.
Application : IHC-P
Reactivity : Pig
Masita Silviana N et al. Turk J Pharm Sci 2021; 18 (1) : 28-33 Masticatory Functional Load Increases the mRNA Expression Levels of ACTN2 and ACTN3 and the Protein Expression of α-Actinin-2 in Rat Masseter Muscle.
Application : WB
Reactivity : Rat
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REVIEW

alpha Actinin 2 antibody [N1N3] Cat. No. GTX103219

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-alpha Actinin 2 antibody [N1N3] used in Western Blot (WB). GTX103219
Date : Anonymous submitted on 14-Dec-2021
Score :
Application Tested : WB
Sample Species : Rat
Sample : rat skeletal muscle tissue
Amount used : 2.5-10μg
Blocking : 3% skim milk, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 12Hr
Anti-alpha Actinin 2 antibody [N1N3] used in Western Blot (WB). GTX103219
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PeerJ in 2018.PMID: 30065891
Submit a Review
Application Reference
Rodriguez Garcia M et al. Int J Mol Sci 2023; 24 (19) Disruption of Z-Disc Function Promotes Mechanical Dysfunction in Human Myocardium: Evidence for a Dual Myofilament Modulatory Role by Alpha-Actinin 2.
Boimpoundi Eunice Flavie Ouali et al. Biochem Biophys Rep 2021; 26:101019 Beta-agonist drugs modulate the proliferation and differentiation of skeletal muscle cells in vitro.
Application : WB
Reactivity : Mouse
Cowan J et al. Sci Rep 2021; 11 (1):6293 Regulation of cardiomyocyte DNA damage and cell death by the type 2A protein phosphatase regulatory protein alpha4.
Weber B et al. Sci Rep 2021; 11 (1):8503 Early myocardial damage (EMD) and valvular dysfunction after femur fracture in pigs.
Application : IHC-P
Reactivity : Pig
Masita Silviana N et al. Turk J Pharm Sci 2021; 18 (1):28-33 Masticatory Functional Load Increases the mRNA Expression Levels of ACTN2 and ACTN3 and the Protein Expression of α-Actinin-2 in Rat Masseter Muscle.
Application : WB
Reactivity : Rat
Li H et al. Cells 2019; 8 (9) PFN2a Suppresses C2C12 Myogenic Development by Inhibiting Proliferation and Promoting Apoptosis via the p53 Pathway.
Application : WB
Reactivity : Human
Foertsch S et al. Brain Behav Immun 2019; (Epub) Sensory contact to the stressor prevents recovery from structural and functional heart damage following psychosocial trauma.
Application : WB
Reactivity : Mouse
Wang L et al. Med Sci Monit 2019; 25:2435-2444 Qishen Yiqi Dropping Pills Ameliorates Doxorubicin-induced Cardiotoxicity in Mice via Enhancement of Cardiac Angiogenesis.
Application : IHC
Reactivity : Mouse
Roberts MD et al. PeerJ 2018; 6:e5338 Skeletal muscle mitochondrial volume and myozenin-1 protein differences exist between high versus low anabolic responders to resistance training.
Chang YC et al. Journal of Food and Drug Analysis 2018; (Epub) resveratrol protects muscle cells against palmitate-induced cellular senescence and * resistance through ameliorating autophagic flux.
Application : WB
Reactivity : Mouse
Kalbitz M et al. Sci Rep 2017; 7 (1):17861 Cardiac Depression in Pigs after Multiple Trauma - Characterization of Posttraumatic Structural and Functional Alterations.
Application : IHC-P
Reactivity : Pig
Yeh CJ et al. Sci Rep 2017; 7 (1):15599 The effects of artocarpin on wound healing: in vitro and in vivo studies.
Application : WB
Reactivity : Human
Jin JK et al. Circ Res 2016; ATF6 Decreases Myocardial Ischemia/Reperfusion Damage and Links ER Stress and Oxidative Stress Signaling Pathways in the Heart.
Application : ICC/IF
Reactivity : Rat
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169