APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:10000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
NIH-3T3 , A431 , H1299 , Rat heart
Product Note
Based on sequence homology, this antibody may cross react with ACTN1 protein.
Predict Reactivity
Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris-Glycine (pH7), 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human alpha Actinin 2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1949577
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
actinin alpha 2 , CMD1AA , CMH23
Cellular Localization
Cytoplasm , myofibril , sarcomere , Z-disk
Background
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX111167 WB Image
Mouse tissue extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N2C1], Internal (GTX111167) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX111167 IHC-P Image
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using alpha Actinin 2 antibody [N2C1], Internal (GTX111167) at 1:300 dilution.
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GTX111167 WB Image
Sample (10 μg of whole cell lysate) A: adult zebrafish 7.5% SDS PAGE GTX111167 diluted at 1:3000
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GTX111167 IHC-P Image
alpha Actinin 2 antibody [N2C1], Internal detects alpha Actinin 2 protein at cytoplasm and membrane on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. alpha Actinin 2 antibody [N2C1], Internal (GTX111167) diluted at 1:250.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX111167 WB Image
alpha Actinin 2 antibody detects alpha Actinin 2 protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody (GTX111167) diluted at 1:5000.
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GTX111167 WB Image
Sample (30 ug of whole cell lysate) A: A431 (GTX27909) B: H1299 7.5% SDS PAGE GTX111167 diluted at 1:1000
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GTX111167 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using actinin, alpha 2(GTX111167) antibody at 1:200 dilution.
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GTX111167 WB Image
Sample (30 ug of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE GTX111167 diluted at 1:3000
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REFERENCE
There are currently no references for alpha Actinin 2 antibody [N2C1], Internal (GTX111167). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for alpha Actinin 2 antibody [N2C1], Internal (GTX111167). Be the first to share your experience with this product.