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alpha Actinin 2 antibody [N2C1], Internal

Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167
Anti-alpha Actinin 2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111167
Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167
Anti-alpha Actinin 2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111167
Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167
Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167
Anti-alpha Actinin 2 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111167
Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167

Cat. No. GTX111167

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all alpha Actinin 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

104 kDa. ( Note )

Positive Control

NIH-3T3 , A431 , H1299 , Rat heart

Product Note

Based on sequence homology, this antibody may cross react with ACTN1 protein.

Predict Reactivity

Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Actinin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949577

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

actinin alpha 2 , CMD1AA , CMH23

Cellular Localization

Cytoplasm , myofibril , sarcomere , Z-disk

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. [provided by RefSeq]

Database

  • Gene ID: 88 ACTN2
  • UniProt: P35609 ACTN2

Research Area

DATA IMAGES

Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167

GTX111167 WB Image

Mouse tissue extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N2C1], Internal (GTX111167) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111167

GTX111167 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using alpha Actinin 2 antibody [N2C1], Internal (GTX111167) at 1:300 dilution.

Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167

GTX111167 WB Image

Sample (10 μg of whole cell lysate)
A: adult zebrafish
7.5% SDS PAGE
GTX111167 diluted at 1:3000

Anti-alpha Actinin 2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111167

GTX111167 IHC-P Image

alpha Actinin 2 antibody [N2C1], Internal detects alpha Actinin 2 protein at cytoplasm and membrane on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
alpha Actinin 2 antibody [N2C1], Internal (GTX111167) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167

GTX111167 WB Image

alpha Actinin 2 antibody detects alpha Actinin 2 protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody (GTX111167) diluted at 1:5000.

Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167

GTX111167 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
7.5% SDS PAGE
GTX111167 diluted at 1:1000

Anti-alpha Actinin 2 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111167

GTX111167 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using actinin, alpha 2(GTX111167) antibody at 1:200 dilution.

Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167

GTX111167 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
GTX111167 diluted at 1:3000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169