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alpha Actinin 3 antibody

Anti-alpha Actinin 3 antibody used in Western Blot (WB). GTX103216
Anti-alpha Actinin 3 antibody used in Western Blot (WB). GTX103216
Anti-alpha Actinin 3 antibody used in Western Blot (WB). GTX103216
Anti-alpha Actinin 3 antibody used in Immunoprecipitation (IP). GTX103216
Anti-alpha Actinin 3 antibody used in Western Blot (WB). GTX103216

Cat. No. GTX103216

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all alpha Actinin 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

Positive Control

NIH-3T3 , JC , PC-12 , 293T , A431 , HeLa , HepG2

Predict Reactivity

Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Actinin 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10732171

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actinin alpha 3 (gene/pseudogene) , ACTN3D

Background

Alpha-actinin is an actin-binding protein with multiple roles in different cell types. This gene expression is limited to skeletal muscle. It is localized to the Z-disc and analogous dense bodies, where it helps to anchor the myofibrillar actin filaments [provided by RefSeq]

Database

  • Gene ID: 89 ACTN3
  • UniProt: Q08043 ACTN3

Research Area

DATA IMAGES

Anti-alpha Actinin 3 antibody used in Western Blot (WB). GTX103216

GTX103216 WB Image

Sample (30 μg of whole cell lysate)
A: whole zebrafish
7.5% SDS PAGE
GTX103216 diluted at 1:1000

Anti-alpha Actinin 3 antibody used in Western Blot (WB). GTX103216

GTX103216 WB Image

Sample (30 ug of whole cell lysate)
A: PC-12
7.5% SDS PAGE
GTX103216 diluted at 1:10000

Anti-alpha Actinin 3 antibody used in Western Blot (WB). GTX103216

GTX103216 WB Image

alpha Actinin 3 antibody detects alpha Actinin 3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 3 antibody (GTX103216) diluted by 1:10000.

Anti-alpha Actinin 3 antibody used in Immunoprecipitation (IP). GTX103216

GTX103216 IP Image

Immunoprecipitation of alpha Actinin 3 protein from HeLa whole cell extracts using 5 μg of alpha Actinin 3 antibody (GTX103216).
Western blot analysis was performed using alpha Actinin 3 antibody (GTX103216).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-alpha Actinin 3 antibody used in Western Blot (WB). GTX103216

GTX103216 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
7.5% SDS PAGE
GTX103216 diluted at 1:10000

REFERENCE

Application Reference

Masita Silviana N et al. Turk J Pharm Sci 2021; 18 (1) : 28-33 Masticatory Functional Load Increases the mRNA Expression Levels of ACTN2 and ACTN3 and the Protein Expression of α-Actinin-2 in Rat Masseter Muscle.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169