GeneTex
United States (US)

alpha Actinin 3 antibody

GTX103216_40471_20141017_WB_19011506_557.jpg
GTX103216_40471_20150529_IP_19011506_933.jpg
GTX103216_40471_WB_R_19011506_258.jpg
GTX103216_40471_WB_M_19011506_359.jpg

Cat No. GTX103216

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , NIH-3T3 , JC , PC-12

Predict Reactivity

Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Actinin 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MGC117002 antibody, MGC117005 antibody, ACTN3 antibody, F-actin cross-linking protein antibody, alpha-actinin-3 antibody, alpha-actinin skeletal muscle antibody, "actinin, alpha 3 antibody"

Background

Alpha-actinin is an actin-binding protein with multiple roles in different cell types. This gene expression is limited to skeletal muscle. It is localized to the Z-disc and analogous dense bodies, where it helps to anchor the myofibrillar actin filaments [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103216_40471_20141017_WB_19011506_557.jpg

GTX103216 WB Image

alpha Actinin 3 antibody detects alpha Actinin 3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 3 antibody (GTX103216) diluted by 1:10000.

GTX103216_40471_20150529_IP_19011506_933.jpg

GTX103216 IP Image

Immunoprecipitation of alpha Actinin 3 protein from HeLa whole cell extracts using 5 μg of alpha Actinin 3 antibody (GTX103216).
Western blot analysis was performed using alpha Actinin 3 antibody (GTX103216).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX103216_40471_WB_R_19011506_258.jpg

GTX103216 WB Image

Sample (30 ug of whole cell lysate)
A: PC-12
7.5% SDS PAGE
GTX103216 diluted at 1:10000

GTX103216_40471_WB_M_19011506_359.jpg

GTX103216 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
7.5% SDS PAGE
GTX103216 diluted at 1:10000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169