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alpha Actinin 3 antibody

Anti-alpha Actinin 3 antibody used in Western Blot (WB). GTX54908
Anti-alpha Actinin 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54908

Cat. No. GTX54908

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all alpha Actinin 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of alpha Actinin 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actinin alpha 3 (gene/pseudogene) , ACTN3D

Background

This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status. [provided by RefSeq, Feb 2014]

Database

  • Gene ID: 89 ACTN3
  • UniProt: Q08043 ACTN3

Research Area

DATA IMAGES

Anti-alpha Actinin 3 antibody used in Western Blot (WB). GTX54908

GTX54908 WB Image

WB analysis of HeLa (A), mouse muscle (B), rat muscle (C) whole cell lysates using GTX54908 alpha Actinin 3 antibody.

Anti-alpha Actinin 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54908

GTX54908 ICC/IF Image

ICC/IF analysis of formalin-fixed HeLa cells using GTX54908 alpha Actinin 3 antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399