GeneTex
United States (US)

alpha Actinin 4 antibody [7H6]

GTX15648_1024_IHC-P_19011519_143.jpg
GTX15648_1025_IHC-P_19011519_270.jpg
GTX15648_1026_IHC-P_19011519_419.jpg
GTX15648_2094_WB_19011519_807.jpg

Cat No. GTX15648

Host Mouse
Clonality Monoclonal
Clone Name 7H6
Isotype IgG
Application WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 2 μg/mL
ICC/IF 4 μg/mL
IHC-P 4 μg/ml
FACS 1μg per 10⁶ cells
ELISA Assay dependent
Not tested in other applications.

Calculated MW

105 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues A(884) P Y Q G P D A V P G A L D(897) of human alpha Actinin 4.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Actinin Alpha 4,Actinin-4,Fsgs,Fsgs1,Actn4

Cellular Localization

Cell junction,Cytoplasm,Cytoskeleton,Nucleus,Stress fiber

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX15648_1024_IHC-P_19011519_143.jpg

GTX15648 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX15648 alpha Actinin 4 antibody [7H6].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

GTX15648_1025_IHC-P_19011519_270.jpg

GTX15648 IHC-P Image

IHC-P analysis of human kidney tissue using GTX15648 alpha Actinin 4 antibody [7H6].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

GTX15648_1026_IHC-P_19011519_419.jpg

GTX15648 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX15648 alpha Actinin 4 antibody [7H6].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

GTX15648_2094_WB_19011519_807.jpg

GTX15648 WB Image

WB analysis of samples using GTX15648 alpha Actinin 4 antibody [7H6].

REFERENCE