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alpha Actinin 4 antibody [7H6]

Anti-alpha Actinin 4 antibody [7H6] used in IHC (Paraffin sections) (IHC-P). GTX15648
Anti-alpha Actinin 4 antibody [7H6] used in IHC (Paraffin sections) (IHC-P). GTX15648
Anti-alpha Actinin 4 antibody [7H6] used in Western Blot (WB). GTX15648
Anti-alpha Actinin 4 antibody [7H6] used in IHC (Paraffin sections) (IHC-P). GTX15648

Cat. No. GTX15648

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7H6

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all alpha Actinin 4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 μg/mL
ICC/IF 4 μg/mL
IHC-P 4 μg/ml
FACS 1μg per 10⁶ cells
ELISA Assay dependent
Not tested in other applications.

Calculated MW

105 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues A(884) P Y Q G P D A V P G A L D(897) of human alpha Actinin 4.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_2887521

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actinin alpha 4 , ACTININ-4 , FSGS , FSGS1

Cellular Localization

Nucleus,Cytoplasm,Cell junction

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq, Jul 2008]

Database

  • Gene ID: 81 ACTN4
  • UniProt: O43707 ACTN4

Research Area

DATA IMAGES

Anti-alpha Actinin 4 antibody [7H6] used in IHC (Paraffin sections) (IHC-P). GTX15648

GTX15648 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX15648 alpha Actinin 4 antibody [7H6].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-alpha Actinin 4 antibody [7H6] used in IHC (Paraffin sections) (IHC-P). GTX15648

GTX15648 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX15648 alpha Actinin 4 antibody [7H6].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-alpha Actinin 4 antibody [7H6] used in Western Blot (WB). GTX15648

GTX15648 WB Image

WB analysis of samples using GTX15648 alpha Actinin 4 antibody [7H6].

Anti-alpha Actinin 4 antibody [7H6] used in IHC (Paraffin sections) (IHC-P). GTX15648

GTX15648 IHC-P Image

IHC-P analysis of human kidney tissue using GTX15648 alpha Actinin 4 antibody [7H6].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

REFERENCE

Application Reference

Lin YH et al. Biomedicines 2020; 8 (11) ACTN4 Mediates SEPT14 Mutation-Induced Sperm Head Defects.
Durcin M et al. J Extracell Vesicles 2017; 6 (1) : 1305677 Characterisation of adipocyte-derived extracellular vesicle subtypes identifies distinct protein and lipid signatures for large and small extracellular vesicles.
Durcin M et al. JEV 2017; (Epub) Characterisation of adipocyte-derived extracellular vesicle subtypes identifies distinct protein and lipid signatures for large and small extracellular vesicles.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399