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alpha Actinin 4 antibody [C2C3], C-term

Orthogonal
Anti-alpha Actinin 4 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101669
Anti-alpha Actinin 4 antibody [C2C3], C-term used in Western Blot (WB). GTX101669
Anti-alpha Actinin 4 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101669
Anti-alpha Actinin 4 antibody [C2C3], C-term used in Western Blot (WB). GTX101669
Anti-alpha Actinin 4 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101669
Anti-alpha Actinin 4 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101669
Anti-alpha Actinin 4 antibody [C2C3], C-term used in Western Blot (WB). GTX101669
Anti-alpha Actinin 4 antibody [C2C3], C-term used in Western Blot (WB). GTX101669

Cat. No. GTX101669

Reference 8
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all alpha Actinin 4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

105 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A375 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , Mouse brain , PC-12

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human alpha Actinin 4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617403

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actinin alpha 4 , ACTININ-4 , FSGS , FSGS1

Cellular Localization

Nucleus , Cytoplasm

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq]

Database

  • Gene ID: 81 ACTN4
  • UniProt: O43707 ACTN4

Research Area

DATA IMAGES

Anti-alpha Actinin 4 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101669

GTX101669 IHC-P Image

alpha Actinin 4 antibody [C2C3], C-term detects alpha Actinin 4 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
alpha Actinin 4 stained by alpha Actinin 4 antibody [C2C3], C-term (GTX101669) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Actinin 4 antibody [C2C3], C-term used in Western Blot (WB). GTX101669

GTX101669 WB Image

alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw264.7 whole cell lysate/extract
G. 30 μg C2C12 whole cell lysate/extract
7.5% SDS-PAGE
alpha Actinin 4 antibody [C2C3], C-term (GTX101669) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 4 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101669

GTX101669 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using alpha Actinin 4(GTX101669) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-alpha Actinin 4 antibody [C2C3], C-term used in Western Blot (WB). GTX101669

GTX101669 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX101669 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 4 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101669

GTX101669 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using alpha Actinin 4(GTX101669) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha Actinin 4 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101669

GTX101669 IHC-P Image

alpha Actinin 4 antibody [C2C3], C-term detects alpha Actinin 4 protein on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
alpha Actinin 4 antibody [C2C3], C-term (GTX101669) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha Actinin 4 antibody [C2C3], C-term used in Western Blot (WB). GTX101669

GTX101669 WB Image

alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
7.5% SDS-PAGE
alpha Actinin 4 antibody [C2C3], C-term (GTX101669) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 4 antibody [C2C3], C-term used in Western Blot (WB). GTX101669

GTX101669 WB Image

alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
E. 30 μg A375 whole cell lysate/extract
7.5% SDS-PAGE
alpha Actinin 4 antibody [C2C3], C-term (GTX101669) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Shu-Hui Yeh et al. Sci Rep 2021; 11 (1) : 15783 Higher senescence associated secretory phenotype and lower defense mediator in urinary extracellular vesicles of elders with and without Parkinson disease.
Application : WB
Reactivity : Human
Ho CH et al. Front Physiol 2021; 12 : 725172 α-Actinin 4 Links Vasopressin Short-Term and Long-Term Regulation of Aquaporin-2 in Kidney Collecting Duct Cells.
Application : WB
Hiraga C et al. Sci Rep 2021; 11 (1) : 3326 Pentapartite fractionation of particles in oral fluids by differential centrifugation.
Application : WB
Takov K et al. J Extracell Vesicles 2019; 8 (1) : 1560809 Comparison of small extracellular vesicles isolated from plasma by ultracentrifugation or size-exclusion chromatography: yield, purity and functional potential.
Application : WB
Reactivity : Rat
Ageta H et al. Nat Commun 2018; 9 (1) : 3936 UBL3 modification influences protein sorting to small extracellular vesicles.
Application : WB
Reactivity : Mouse
Show More +
Submit a Reference

REVIEW

alpha Actinin 4 antibody [C2C3], C-term Cat. No. GTX101669

Rating
( Average 4.5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 4.5 based on 2 users reviews)
Anti-alpha Actinin 4 antibody [C2C3], C-term used in Western Blot (WB). GTX101669
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : WB
Sample Species : Hu, Ms
Figure Note : The data was peer reviewed and published in the 2018 in Nat Commun. PMID: 30258067
Anti-alpha Actinin 4 antibody [C2C3], C-term used in Western Blot (WB). GTX101669
Date : Anonymous submitted on 23-Jan-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : HepG2 cells
Amount used : 30μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
Application Reference
Shu-Hui Yeh et al. Sci Rep 2021; 11 (1):15783 Higher senescence associated secretory phenotype and lower defense mediator in urinary extracellular vesicles of elders with and without Parkinson disease.
Application : WB
Reactivity : Human
Ho CH et al. Front Physiol 2021; 12:725172 α-Actinin 4 Links Vasopressin Short-Term and Long-Term Regulation of Aquaporin-2 in Kidney Collecting Duct Cells.
Application : WB
Hiraga C et al. Sci Rep 2021; 11 (1):3326 Pentapartite fractionation of particles in oral fluids by differential centrifugation.
Application : WB
Takov K et al. J Extracell Vesicles 2019; 8 (1):1560809 Comparison of small extracellular vesicles isolated from plasma by ultracentrifugation or size-exclusion chromatography: yield, purity and functional potential.
Application : WB
Reactivity : Rat
Ageta H et al. Nat Commun 2018; 9 (1):3936 UBL3 modification influences protein sorting to small extracellular vesicles.
Application : WB
Reactivity : Mouse
Durrieu L et al. Blood Adv 2018; 2 (10):1054-1065 Analysis of the thrombotic and fibrinolytic activities of tumor cell-derived extracellular vesicles.
Reactivity : Human
Kowal J et al. Proc Natl Acad Sci U S A 2016; 113 (8):E968-77 Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes.
Application : WB
Reactivity : Human
Choi W et al. J Cell Biol 2016; 213 (2):243-60 Remodeling the zonula adherens in response to tension and the role of afadin in this response.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169