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alpha Actinin 4 antibody [C2C3], C-term

Anti-alpha Actinin 4 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101669
Anti-alpha Actinin 4 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101669
Anti-alpha Actinin 4 antibody [C2C3], C-term used in Western Blot (WB). GTX101669
Anti-alpha Actinin 4 antibody [C2C3], C-term used in Western Blot (WB). GTX101669
Anti-alpha Actinin 4 antibody [C2C3], C-term used in Western Blot (WB). GTX101669
Anti-alpha Actinin 4 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101669
Anti-alpha Actinin 4 antibody [C2C3], C-term used in Western Blot (WB). GTX101669
Anti-alpha Actinin 4 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101669

Cat No. GTX101669

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

105 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A375 , Neuro 2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , mouse brain , PC-12

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human alpha Actinin 4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617403

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

actinin alpha 4 , ACTININ-4 , FSGS , FSGS1

Cellular Localization

Nucleus , Cytoplasm

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq]

Database

  • Gene ID: 81 ACTN4
  • UniProt: O43707 ACTN4

Research Area

DATA IMAGES

Anti-alpha Actinin 4 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101669

GTX101669 IHC-P Image

alpha Actinin 4 antibody [C2C3], C-term detects alpha Actinin 4 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
alpha Actinin 4 stained by alpha Actinin 4 antibody [C2C3], C-term (GTX101669) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Actinin 4 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101669

GTX101669 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using alpha Actinin 4(GTX101669) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-alpha Actinin 4 antibody [C2C3], C-term used in Western Blot (WB). GTX101669

GTX101669 WB Image

alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
E. 30 μg A375 whole cell lysate/extract
7.5% SDS-PAGE
alpha Actinin 4 antibody [C2C3], C-term (GTX101669) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 4 antibody [C2C3], C-term used in Western Blot (WB). GTX101669

GTX101669 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX101669 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 4 antibody [C2C3], C-term used in Western Blot (WB). GTX101669

GTX101669 WB Image

alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw264.7 whole cell lysate/extract
G. 30 μg C2C12 whole cell lysate/extract
7.5% SDS-PAGE
alpha Actinin 4 antibody [C2C3], C-term (GTX101669) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 4 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101669

GTX101669 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using alpha Actinin 4(GTX101669) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha Actinin 4 antibody [C2C3], C-term used in Western Blot (WB). GTX101669

GTX101669 WB Image

alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
7.5% SDS-PAGE
alpha Actinin 4 antibody [C2C3], C-term (GTX101669) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 4 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101669

GTX101669 IHC-P Image

alpha Actinin 4 antibody [C2C3], C-term detects alpha Actinin 4 protein on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
alpha Actinin 4 antibody [C2C3], C-term (GTX101669) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

alpha Actinin 4 antibody [C2C3], C-term Cat No. GTX101669

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 23-Jan-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : HepG2 cells
Amount used : 30μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169