APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , A375 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , Mouse brain , PC-12
Predict Reactivity
Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris-Glycine (pH7), 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.32 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human alpha Actinin 4. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10617403
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
actinin alpha 4 , ACTININ-4 , FSGS , FSGS1
Cellular Localization
Nucleus , Cytoplasm
Background
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX101669 IHC-P Image
alpha Actinin 4 antibody [C2C3], C-term detects alpha Actinin 4 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.alpha Actinin 4 stained by alpha Actinin 4 antibody [C2C3], C-term (GTX101669) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX101669 WB Image
alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by western blot analysis. A. 30 μg Neuro2A whole cell lysate/extract B. 30 μg GL261 whole cell lysate/extract C. 30 μg C8D30 whole cell lysate/extract D. 30 μg NIH-3T3 whole cell lysate/extract E. 30 μg BCL-1 whole cell lysate/extract F. 30 μg Raw264.7 whole cell lysate/extract G. 30 μg C2C12 whole cell lysate/extract 7.5% SDS-PAGE alpha Actinin 4 antibody [C2C3], C-term (GTX101669) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101669 ICC/IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using alpha Actinin 4(GTX101669) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
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GTX101669 WB Image
Sample (50 μg of whole cell lysate) A: Mouse brain 5% SDS PAGE GTX101669 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101669 IHC-P Image
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using alpha Actinin 4(GTX101669) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101669 IHC-P Image
alpha Actinin 4 antibody [C2C3], C-term detects alpha Actinin 4 protein on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. alpha Actinin 4 antibody [C2C3], C-term (GTX101669) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101669 WB Image
alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by western blot analysis. A. 30 μg PC-12 whole cell lysate/extract 7.5% SDS-PAGE alpha Actinin 4 antibody [C2C3], C-term (GTX101669) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101669 WB Image
alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract E. 30 μg A375 whole cell lysate/extract 7.5% SDS-PAGE alpha Actinin 4 antibody [C2C3], C-term (GTX101669) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
REVIEW
alpha Actinin 4 antibody [C2C3], C-term
Cat. No. GTX101669
Rating
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( Average 4.5 based on 2 users reviews)
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Application
Western Blot(WB)
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( Average 4.5 based on 2 users reviews)
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Date :
Anonymous submitted on 24-Jul-2023
Application Tested :
WB
Sample Species :
Hu, Ms
Figure Note :
The data was peer reviewed and published in the 2018 in Nat Commun.
PMID: 30258067
Date :
Anonymous submitted on 23-Jan-2015
Application Tested :
WB
Sample Species :
Hu
Sample :
HepG2 cells
Amount used :
30μg
Blocking :
5% milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr