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alpha Actinin 4 antibody [N1N2], N-term

Anti-alpha Actinin 4 antibody [N1N2], N-term used in Western Blot (WB). GTX113116
Anti-alpha Actinin 4 antibody [N1N2], N-term used in Western Blot (WB). GTX113116
Anti-alpha Actinin 4 antibody [N1N2], N-term used in Western Blot (WB). GTX113116
Anti-alpha Actinin 4 antibody [N1N2], N-term used in Western Blot (WB). GTX113116
Anti-alpha Actinin 4 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113116

Cat. No. GTX113116

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all alpha Actinin 4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

105 kDa. ( Note )

Positive Control

A431 , NIH-3T3 , JC , PC-12 , 293T , A431 , HeLa , HepG2

Predict Reactivity

Zebrafish, Drosophila, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human alpha Actinin 4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11173447

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actinin alpha 4 , ACTININ-4 , FSGS , FSGS1

Cellular Localization

Nucleus , Cytoplasm

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq]

Database

  • Gene ID: 81 ACTN4
  • UniProt: O43707 ACTN4

Research Area

DATA IMAGES

Anti-alpha Actinin 4 antibody [N1N2], N-term used in Western Blot (WB). GTX113116

GTX113116 WB Image

alpha Actinin 4 antibody detects alpha Actinin 4 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 4 antibody (GTX113116) diluted by 1:1000.

Anti-alpha Actinin 4 antibody [N1N2], N-term used in Western Blot (WB). GTX113116

GTX113116 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
7.5% SDS PAGE
GTX113116 diluted at 1:1000

Anti-alpha Actinin 4 antibody [N1N2], N-term used in Western Blot (WB). GTX113116

GTX113116 WB Image

alpha Actinin 4 antibody [N1N2], N-term detects ACTN4 protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
7.5 % SDS-PAGE
alpha Actinin 4 antibody [N1N2], N-term (GTX113116) dilution: 1:1000

Anti-alpha Actinin 4 antibody [N1N2], N-term used in Western Blot (WB). GTX113116

GTX113116 WB Image

Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
GTX113116 diluted at 1:1000

Anti-alpha Actinin 4 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113116

GTX113116 ICC/IF Image

alpha Actinin 4 antibody [N1N2], N-term detects ACTN4 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: ACTN4 protein stained by alpha Actinin 4 antibody [N1N2], N-term (GTX113116) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169