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alpha Actinin 1 antibody [0.T.02]

Anti-alpha Actinin 1 antibody [0.T.02] used in Western Blot (WB). GTX18061

Cat. No. GTX18061

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 0.T.02

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Human, Mouse, Rat, Xenopus
Package
100 μg ($429)
See all alpha Actinin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5-2μg/ml
ICC/IF 15μg/ml (fixed with methanol)
IHC-Fr 15μg/ml
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

Product Note

Recognizes human alpha-Actinin 1 isoform (non-muscle) at 100kD.

PROPERTIES

Form

Liquid

Buffer

0.02M PBS, 0.25M NaCl

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human alpha-Actinin

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_423052

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ACTN1 , BDPLT15 , actinin alpha 1 , alpha Actinin 1

Cellular Localization

Cytoplasm,Cytoskeleton,Myofibril,Sarcomere,Z line,Cell membrane,Cell junction,Cell projection,Ruffle

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

  • Gene ID: 87 ACTN1
  • UniProt: P12814 ACTN1

Research Area

DATA IMAGES

Anti-alpha Actinin 1 antibody [0.T.02] used in Western Blot (WB). GTX18061

GTX18061 WB Image

WB analysis of A431 cell lysate using GTX18061 alpha Actinin 1 antibody [0.T.02].

REFERENCE

Application Reference

Has C et al. Am J Pathol 2009; 175 (4) : 1442-52 Kindlin-1 Is required for RhoGTPase-mediated lamellipodia formation in keratinocytes.
Application : WB
Reactivity : Human
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 429