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alpha Actinin antibody [7A4]

KO/KD Overexpression
Anti-alpha Actinin antibody [7A4] used in IHC (Paraffin sections) (IHC-P). GTX84959
Anti-alpha Actinin antibody [7A4] used in IHC (Paraffin sections) (IHC-P). GTX84959
Anti-alpha Actinin antibody [7A4] used in Western Blot (WB). GTX84959
Anti-alpha Actinin antibody [7A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84959
Anti-alpha Actinin antibody [7A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84959
Anti-alpha Actinin antibody [7A4] used in IHC (Paraffin sections) (IHC-P). GTX84959
Anti-alpha Actinin antibody [7A4] used in Western Blot (WB). GTX84959
Anti-alpha Actinin antibody [7A4] used in Western Blot (WB). GTX84959
Anti-alpha Actinin antibody [7A4] used in IHC (Paraffin sections) (IHC-P). GTX84959
Anti-alpha Actinin antibody [7A4] used in Western Blot (WB). GTX84959
Anti-alpha Actinin antibody [7A4] used in IHC (Paraffin sections) (IHC-P). GTX84959

Cat. No. GTX84959

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7A4

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($519)
See all alpha Actinin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant protein fragment corresponding to amino acids 650-893 of human alpha-actinin (NP_001093) produced in E.coli.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_10727698

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actinin alpha 1 , BDPLT15

Cellular Localization

Cytoplasm, cytoskeleton,Cell membrane,Cell junction

Background

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

Database

  • Gene ID: 87 ACTN1
  • UniProt: P12814 ACTN1

Research Area

DATA IMAGES

Anti-alpha Actinin antibody [7A4] used in IHC (Paraffin sections) (IHC-P). GTX84959

GTX84959 IHC-P Image

IHC-P analysis of human liver tissue using GTX84959 alpha Actinin antibody [7A4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-alpha Actinin antibody [7A4] used in IHC (Paraffin sections) (IHC-P). GTX84959

GTX84959 IHC-P Image

IHC-P analysis of human bladder tissue using GTX84959 alpha Actinin antibody [7A4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-alpha Actinin antibody [7A4] used in Western Blot (WB). GTX84959

GTX84959 WB Image

WB analysis of various cell lines using GTX84959 alpha Actinin antibody [7A4].
Loading : 10 ug per lane
Dilution : 1:200

Anti-alpha Actinin antibody [7A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84959

GTX84959 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX84959 alpha Actinin antibody [7A4].

Anti-alpha Actinin antibody [7A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84959

GTX84959 ICC/IF Image

ICC/IF analysis of HeLa cells transiently transfected with alpha Actinin plasmid using GTX84959 alpha Actinin antibody [7A4].

Anti-alpha Actinin antibody [7A4] used in IHC (Paraffin sections) (IHC-P). GTX84959

GTX84959 IHC-P Image

IHC-P analysis of human breast tissue using GTX84959 alpha Actinin antibody [7A4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-alpha Actinin antibody [7A4] used in Western Blot (WB). GTX84959

GTX84959 WB Image

WB analysis of various samples using GTX84959 alpha Actinin antibody [7A4].
Loading : 10 ug per lane
Dilution : 1:200

Anti-alpha Actinin antibody [7A4] used in Western Blot (WB). GTX84959

GTX84959 WB Image

KO/KD

WB analysis of wild type and alpha Actinin knock out HeLa cell lysate(10μg per lane) using GTX84959 alpha Actinin antibody [7A4].
Dilution : 1:500

Anti-alpha Actinin antibody [7A4] used in IHC (Paraffin sections) (IHC-P). GTX84959

GTX84959 IHC-P Image

IHC-P analysis of human kidney tissue using GTX84959 alpha Actinin antibody [7A4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-alpha Actinin antibody [7A4] used in Western Blot (WB). GTX84959

GTX84959 WB Image

Overexpression

WB analysis of HEK293T cells transfected with alpha Actinin plasmid (Right) or empty vector (Left) for 48 hrs using GTX84959 alpha Actinin antibody [7A4].
Loading : 5 ug per lane

Anti-alpha Actinin antibody [7A4] used in IHC (Paraffin sections) (IHC-P). GTX84959

GTX84959 IHC-P Image

IHC-P analysis of human ovary tissue using GTX84959 alpha Actinin antibody [7A4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

Application Reference

Rojas C et al. Br J Pharmacol 2019; (Epub) A novel and potent brain penetrant inhibitor of extracellular vesicle release.
Application : WB
Reactivity : Human
Foertsch S et al. Brain Behav Immun 2019; (Epub) Sensory contact to the stressor prevents recovery from structural and functional heart damage following psychosocial trauma.
Application : IHC-P
Reactivity : Mouse
Liang C et al. J Appl Physiol (1985) 2019; 126 (4) : 799-809 Functional and biochemical responses of skeletal muscle following a moderate degree of systemic iron loading in mice.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519