alpha Actinin antibody [HL1692]

Cat. No. GTX637291

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1692
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

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Datasheet

View product citations for antibody GTX637291 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	Assay dependent
IHC-P	Assay dependent

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragmemt of human alpha Actinin

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actinin alpha 4 , actinin alpha 1 , actinin alpha 2 , actinin alpha 3 (gene/pseudogene) , ACTININ-4 , FSGS , FSGS1 , BDPLT15 , CMD1AA , CMH23 , ACTN3D

Cellular Localization

Cytoplasm, cytoskeleton,Cytoplasm, myofibril, sarcomere, Z line,Cell membrane,Cell junction,Nucleus,Cytoplasm

Database

Gene ID: 81 ACTN4
Gene ID: 87 ACTN1
Gene ID: 88 ACTN2
Gene ID: 89 ACTN3
UniProt: Q08043 ACTN4
UniProt: P12814 ACTN1
UniProt: O43707 ACTN2
UniProt: P35609 ACTN3

Research Area

DATA IMAGES

GTX637291 ICC/IF Image

alpha Actinin 2 antibody [HL1692] detects alpha Actinin 2 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: alpha Actinin 2 stained by alpha Actinin 2 antibody [HL1692] (GTX637291) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX637291 IHC-P Image

alpha Actinin 2 antibody [HL1692] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
alpha Actinin 2 stained by alpha Actinin 2 antibody [HL1692] (GTX637291) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX637291 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with alpha Actinin antibody [HL1692] (GTX637291) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations

alpha Actinin antibody [HL1692]

Cat. No. GTX637291

Host

Clonality

Clone Name

Isotype

Application

Reactivity