APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Mouse liver , Jurkat , Raji , NCI-H929
Predict Reactivity
Rat, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.96 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human alpha Adducin. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1949589
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
adducin 1 , ADDA
Cellular Localization
Cytoplasm , cytoskeleton , Cell membrane
Background
Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-alpha Adducin antibody [N1N2], N-term used in IHC (Whole mount) (IHC-Wm). GTX113118](/templates/default/js/lightbox/images/loading.gif "Anti-alpha Adducin antibody [N1N2], N-term used in IHC (Whole mount) (IHC-Wm). GTX113118")
|
GTX113118 IHC-Wm Image
alpha Adducin antibody [N1N2], N-term detects alpha Adducin protein at nucleus on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
alpha Adducin antibody [N1N2], N-term (GTX113118) dilution: 1:200.
|
|
|
GTX113118 IHC-P Image
Immunohistochemical analysis of paraffin-embedded SG xenograft , using Alpha-adducin(GTX113118) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX113118 WB Image
alpha Adducin antibody [N1N2], N-term detects alpha Adducin protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
7.5 % SDS-PAGE
alpha Adducin antibody [N1N2], N-term (GTX113118) dilution: 1:1000
|
|
|
GTX113118 WB Image
Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX113118 diluted at 1:1000
|
REFERENCE
There are currently no references for alpha Adducin antibody [N1N2], N-term (GTX113118). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for alpha Adducin antibody [N1N2], N-term (GTX113118). Be the first to share your experience with this product.
![Anti-alpha Adducin antibody [N1N2], N-term used in IHC (Whole mount) (IHC-Wm). GTX113118](/upload/website/prouct_img/small/GTX113118/GTX113118_40086_IHC-Wm_Z_22111423_628.webp "Anti-alpha Adducin antibody [N1N2], N-term used in IHC (Whole mount) (IHC-Wm). GTX113118")
![Anti-alpha Adducin antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113118](/upload/website/prouct_img/small/GTX113118/GTX113118_IHC_w_23060500_158.webp "Anti-alpha Adducin antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113118")
![Anti-alpha Adducin antibody [N1N2], N-term used in Western Blot (WB). GTX113118](/upload/website/prouct_img/small/GTX113118/GTX113118_40086_WB_w_23060500_666.webp "Anti-alpha Adducin antibody [N1N2], N-term used in Western Blot (WB). GTX113118")
![Anti-alpha Adducin antibody [N1N2], N-term used in Western Blot (WB). GTX113118](/upload/website/prouct_img/small/GTX113118/GTX113118_40086_WB_M_liver_w_23060500_155.webp "Anti-alpha Adducin antibody [N1N2], N-term used in Western Blot (WB). GTX113118")
![Anti-alpha Adducin antibody [N1N2], N-term used in IHC (Whole mount) (IHC-Wm). GTX113118](/upload/website/prouct_img/normal/GTX113118/GTX113118_40086_IHC-Wm_Z_22111423_628.webp "Anti-alpha Adducin antibody [N1N2], N-term used in IHC (Whole mount) (IHC-Wm). GTX113118")
![Anti-alpha Adducin antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113118](/upload/website/prouct_img/normal/GTX113118/GTX113118_IHC_w_23060500_158.webp "Anti-alpha Adducin antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113118")
![Anti-alpha Adducin antibody [N1N2], N-term used in Western Blot (WB). GTX113118](/upload/website/prouct_img/normal/GTX113118/GTX113118_40086_WB_w_23060500_666.webp "Anti-alpha Adducin antibody [N1N2], N-term used in Western Blot (WB). GTX113118")
![Anti-alpha Adducin antibody [N1N2], N-term used in Western Blot (WB). GTX113118](/upload/website/prouct_img/normal/GTX113118/GTX113118_40086_WB_M_liver_w_23060500_155.webp "Anti-alpha Adducin antibody [N1N2], N-term used in Western Blot (WB). GTX113118")