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alpha Adducin antibody

Anti-alpha Adducin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101574
Anti-alpha Adducin antibody used in IHC (Paraffin sections) (IHC-P). GTX101574
Anti-alpha Adducin antibody used in Western Blot (WB). GTX101574
Anti-alpha Adducin antibody used in IHC (Paraffin sections) (IHC-P). GTX101574

Cat. No. GTX101574

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all alpha Adducin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Positive Control

Jurkat , Raji

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Adducin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885231

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adducin 1 , ADDA

Cellular Localization

Cytoplasm , cytoskeleton , Cell membrane

Background

Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-alpha Adducin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101574

GTX101574 ICC/IF Image

alpha Adducin antibody detects alpha Adducin protein at membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: alpha Adducin protein stained by alpha Adducin antibody (GTX101574) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-alpha Adducin antibody used in IHC (Paraffin sections) (IHC-P). GTX101574

GTX101574 IHC-P Image

alpha Adducin antibody detects alpha Adducin protein at membrane and cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
alpha Adducin antibody (GTX101574) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha Adducin antibody used in Western Blot (WB). GTX101574

GTX101574 WB Image

alpha Adducin antibody detects alpha Adducin protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
7.5% SDS-PAGE
alpha Adducin antibody (GTX101574) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Adducin antibody used in IHC (Paraffin sections) (IHC-P). GTX101574

GTX101574 IHC-P Image

alpha Adducin antibody detects alpha Adducin protein at membrane and cytosol on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
alpha Adducin antibody (GTX101574) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169