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alpha Adducin antibody

Anti-alpha Adducin antibody used in IHC (Whole mount) (IHC-Wm). GTX101600
Anti-alpha Adducin antibody used in IHC (Paraffin sections) (IHC-P). GTX101600
Anti-alpha Adducin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101600
Anti-alpha Adducin antibody used in Western Blot (WB). GTX101600
Anti-alpha Adducin antibody used in IHC (Paraffin sections) (IHC-P). GTX101600

Cat. No. GTX101600

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all alpha Adducin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Positive Control

JurKat

Predict Reactivity

Rat, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.02 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Adducin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036173

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adducin 1 , ADDA

Cellular Localization

Cytoplasm , cytoskeleton , Cell membrane

Background

Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-alpha Adducin antibody used in IHC (Whole mount) (IHC-Wm). GTX101600

GTX101600 IHC-Wm Image

alpha Adducin antibody detects alpha Adducin protein on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
alpha Adducin antibody (GTX101600) dilution: 1:200.
Anti-alpha Adducin antibody used in IHC (Paraffin sections) (IHC-P). GTX101600

GTX101600 IHC-P Image

alpha Adducin antibody detects alpha Adducin protein at membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
alpha Adducin antibody (GTX101600) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha Adducin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101600

GTX101600 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using alpha Adducin(GTX101600) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-alpha Adducin antibody used in Western Blot (WB). GTX101600

GTX101600 WB Image

Sample (30 ug of whole cell lysate)
A: Jurakt
7.5% SDS PAGE
GTX101600 diluted at 1:1000

Anti-alpha Adducin antibody used in IHC (Paraffin sections) (IHC-P). GTX101600

GTX101600 IHC-P Image

alpha Adducin antibody detects alpha Adducin protein at membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
alpha Adducin antibody (GTX101600) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Brieno-Enriquez MA et al. PLoS One 2017; 12 (10) : e0185780 NIMA-related kinase 1 (NEK1) regulates meiosis I spindle assembly by altering the balance between α-Adducin and Myosin X.
Application : ICC/IF
Reactivity : Mouse
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : ICC/IF
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169