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alpha Adducin (phospho Ser726) antibody

Anti-alpha Adducin (phospho Ser726) antibody used in Western Blot (WB). GTX50230
Anti-alpha Adducin (phospho Ser726) antibody used in IHC (Paraffin sections) (IHC-P). GTX50230
Anti-alpha Adducin (phospho Ser726) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50230

Cat. No. GTX50230

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all alpha Adducin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 726 (T-P-S(p)-F-L) derived from human alpha Adducin.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adducin 1 , ADDA

Cellular Localization

Cytoplasm, cytoskeleton

Background

Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, and gamma). Adducin acts as a heterodimer of the related alpha, beta, or gamma subunits. The protein encoded by this gene represents the alpha subunit. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-alpha Adducin (phospho Ser726) antibody used in Western Blot (WB). GTX50230

GTX50230 WB Image

WB analysis of extracts from HeLa cells using GTX50230 alpha Adducin (phospho Ser726) antibody with or without blocking peptide.

Anti-alpha Adducin (phospho Ser726) antibody used in IHC (Paraffin sections) (IHC-P). GTX50230

GTX50230 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX50230 alpha Adducin (phospho Ser726) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-alpha Adducin (phospho Ser726) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50230

GTX50230 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50230 alpha Adducin (phospho Ser726) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399