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alpha Adducin (phospho Ser726) antibody

Anti-alpha Adducin (phospho Ser726) antibody used in Western Blot (WB). GTX52323

Cat No. GTX52323

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC

Reactivity

Human, Mouse, Rat
Package
100 μg ($319)

APPLICATION

Application Note

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Calculated MW

81 kDa. ( Note )

Specificity/Sensitivity

ADD1

PROPERTIES

Form

Liquid

Buffer

phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human ADD1 around the phosphorylation site of Ser726

Purification

Antigen affinity purification

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Adducin 1 , Adda , Add1

Background

Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-alpha Adducin (phospho Ser726) antibody used in Western Blot (WB). GTX52323

GTX52323 WB Image

Western blot analysis of ADD1 phosphorylation expression in Forskolin treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf