alpha B Crystallin antibody [3C2]

Cat. No. GTX84661

Host	Mouse
Clonality	Monoclonal
Clone Name	3C2
Isotype	IgG1
Application	WB, IHC-P, FACS, IP
Reactivity	Human, Monkey
Package	100 μl ($519)

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Datasheet
Document

View product citations for antibody GTX84661 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:5000
IHC-P	1:50
FACS	1:100
IP	2ug/500ul

Not tested in other applications.

Calculated MW

20 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human CRYAB (NP_001876) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

crystallin alpha B , CMD1II , CRYA2 , CTPP2 , CTRCT16 , HEL-S-101 , HSPB5 , MFM2

Cellular Localization

Cytoplasm,Nucleus

Background

May contribute to the transparency and refractive index of the lens.

Database

Gene ID: 1410 CRYAB
UniProt: P02511 CRYAB

Research Area

DATA IMAGES

GTX84661 IHC-P Image

IHC-P analysis of human bladder carcinoma tissue using GTX84661 alpha B Crystallin antibody [3C2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84661 IP Image

IP analysis of DDDDK tagged alpha B Crystallin overexpressed HEK293T lysate using GTX84661 alpha B Crystallin antibody [3C2].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

GTX84661 IHC-P Image

IHC-P analysis of human colon adenocarcinoma tissue using GTX84661 alpha B Crystallin antibody [3C2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84661 WB Image

WB analysis of A549 and COS-7 cell lysate using GTX84661 alpha B Crystallin antibody [3C2].
Loading : 10 ug per lane
Dilution : 1:200

GTX84661 WB Image

WB analysis of HEK293T cells transfected with alpha B Crystallin plasmid (Right) or empty vector (Left) for 48 hrs using GTX84661 alpha B Crystallin antibody [3C2].
Loading : 5 ug per lane

GTX84661 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84661 alpha B Crystallin antibody [3C2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84661 IHC-P Image

IHC-P analysis of human breast adenocarcinoma tissue using GTX84661 alpha B Crystallin antibody [3C2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84661 IHC-P Image

IHC-P analysis of human pancreas carcinoma tissue using GTX84661 alpha B Crystallin antibody [3C2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84661 FACS Image

FACS analysis of HEK293T cells transfected with either alpha B Crystallin plasmid(Red) or empty vector control plasmid(Blue) using GTX84661 alpha B Crystallin antibody [3C2].

GTX84661 IHC-P Image

IHC-P analysis of human lymphoma tissue using GTX84661 alpha B Crystallin antibody [3C2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty

alpha B Crystallin antibody [3C2]

Cat. No. GTX84661

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX84661 IHC-P Image

GTX84661 IP Image

GTX84661 IHC-P Image

GTX84661 WB Image

GTX84661 WB Image

GTX84661 IHC-P Image

GTX84661 IHC-P Image

GTX84661 IHC-P Image

GTX84661 FACS Image

GTX84661 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.