IP analysis of DDDDK tagged alpha B Crystallin overexpressed HEK293T lysate using GTX84659 alpha B Crystallin antibody [6A9].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

FACS analysis of HEK293T cells transfected with either alpha B Crystallin plasmid(Red) or empty vector control plasmid(Blue) using GTX84659 alpha B Crystallin antibody [6A9].

IHC-P analysis of human endometrium tissue using GTX84659 alpha B Crystallin antibody [6A9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

IHC-P analysis of human pancreas tissue using GTX84659 alpha B Crystallin antibody [6A9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

ICC/IF analysis of HT29 cells using GTX84659 alpha B Crystallin antibody [6A9].

ICC/IF analysis of COS7 cells transiently transfected with alpha B Crystallin plasmid using GTX84659 alpha B Crystallin antibody [6A9].

WB analysis of various cell lines using GTX84659 alpha B Crystallin antibody [6A9].
Loading : 35 ug per lane

WB analysis of HEK293T cells transfected with alpha B Crystallin plasmid (Right) or empty vector (Left) for 48 hrs using GTX84659 alpha B Crystallin antibody [6A9].
Loading : 5 ug per lane