IP analysis of DDDDK tagged alpha B Crystallin overexpressed HEK293T lysate using GTX84657 alpha B Crystallin antibody [6D11].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84657 alpha B Crystallin antibody [6D11].
Loading : 10 ug per lane
Dilution : 1:200

WB analysis of HEK293T cells transfected with alpha B Crystallin plasmid (Right) or empty vector (Left) for 48 hrs using GTX84657 alpha B Crystallin antibody [6D11].
Loading : 5 ug per lane

ICC/IF analysis of COS7 cells transiently transfected with alpha B Crystallin plasmid using GTX84657 alpha B Crystallin antibody [6D11].

WB analysis of various cell lines using GTX84657 alpha B Crystallin antibody [6D11].
Loading : 35 ug per lane

IHC-P analysis of human smooth muscle cancer tissue using GTX84657 alpha B Crystallin antibody [6D11].

ICC/IF analysis of HT29 cells using GTX84657 alpha B Crystallin antibody [6D11].

FACS analysis of HEK293T cells transfected with either alpha B Crystallin plasmid(Red) or empty vector control plasmid(Blue) using GTX84657 alpha B Crystallin antibody [6D11].

IHC-P analysis of human thyroid carcinoma tissue using GTX84657 alpha B Crystallin antibody [6D11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.