GeneTex
  • Country / Location Selection

United States (US)

alpha B Crystallin antibody

Anti-alpha B Crystallin antibody used in IHC (Paraffin sections) (IHC-P). GTX66638
Anti-alpha B Crystallin antibody used in Western Blot (WB). GTX66638

Cat No. GTX66638

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($349)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.2, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide, corresponding to amino acids 21-70 of Human Crystallin-αB.

Purification

Purified by antigen-affinity chromatography
From serum

Purity

> 95% (by SDS-PAGE)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

crystallin alpha B , CMD1II , CRYA2 , CTPP2 , CTRCT16 , HEL-S-101 , HSPB5 , MFM2

Cellular Localization

Cytoplasm,Nucleus

Background

Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Database

Research Area

DATA IMAGES

Anti-alpha B Crystallin antibody used in IHC (Paraffin sections) (IHC-P). GTX66638

GTX66638 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX66638 alpha B Crystallin antibody.
Dilution : 1:100

Anti-alpha B Crystallin antibody used in Western Blot (WB). GTX66638

GTX66638 WB Image

WB analysis of various samples using GTX66638 alpha B Crystallin antibody.
Lane2 : PMVEC whole cell lysate(40ug)
Lane3 : A549 whole cell lysate(40ug)
Lane4 : HEK293T whole cell lysate(40ug)
Dilution : 1:500

REFERENCE

There are currently no references for alpha B Crystallin antibody (GTX66638). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for alpha B Crystallin antibody (GTX66638). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 349