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alpha Cardiac Muscle Actin antibody

Anti-alpha Cardiac Muscle Actin antibody used in Western Blot (WB). GTX101900
Anti-alpha Cardiac Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX101900
Anti-alpha Cardiac Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX101900
Anti-alpha Cardiac Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX101900

Cat. No. GTX101900

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Mouse
Package
100 μl ($399),
25 μl ($169)
See all alpha Cardiac Muscle Actin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

NIH-3T3

Predict Reactivity

Human, Rat, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the center region of mouse alpha Cardiac Muscle Actin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949573

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin, alpha, cardiac muscle 1 , Actc-1

Cellular Localization

Cytoplasm , cytoskeleton

Background

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Database

Research Area

DATA IMAGES

Anti-alpha Cardiac Muscle Actin antibody used in Western Blot (WB). GTX101900

GTX101900 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX101900 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Cardiac Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX101900

GTX101900 IHC-P Image

alpha Cardiac Muscle Actin antibody detects alpha Cardiac Muscle Actin protein at cytoplasm on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
alpha Cardiac Muscle Actin antibody (GTX101900) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha Cardiac Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX101900

GTX101900 IHC-P Image

alpha Cardiac Muscle Actin antibody detects alpha Cardiac Muscle Actin protein at cytoplasm on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
alpha Cardiac Muscle Actin antibody (GTX101900) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha Cardiac Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX101900

GTX101900 IHC-P Image

alpha Cardiac Muscle Actin antibody detects alpha Cardiac Muscle Actin protein at cytoplasm on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
alpha Cardiac Muscle Actin antibody (GTX101900) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169