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alpha Catenin antibody [1G5]

Anti-alpha Catenin antibody [1G5] used in Western Blot (WB). GTX17259
Anti-alpha Catenin antibody [1G5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17259
Anti-alpha Catenin antibody [1G5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17259
Anti-alpha Catenin antibody [1G5] used in Western Blot (WB). GTX17259

Cat. No. GTX17259

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1G5

Isotype

 IgG1

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all alpha Catenin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100-1:1000
ICC/IF 10 μg/ml
IP Assay dependent
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

90 kDa MBP/alpha catenin fusion protein purified on amylose column.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

catenin alpha 1 , CAP102 , MDPT2

Cellular Localization

Cell junction

Background

This gene encodes a member of the catenin family of proteins that play an important role in cell adhesion process by connecting cadherins located on the plasma membrane to the actin filaments inside the cell. The encoded mechanosensing protein contains three vinculin homology domains and undergoes conformational changes in response to cytoskeletal tension, resulting in the reconfiguration of cadherin-actin filament connections. Certain mutations in this gene cause butterfly-shaped pigment dystrophy. [provided by RefSeq, May 2016]

Database

Research Area

DATA IMAGES

Anti-alpha Catenin antibody [1G5] used in Western Blot (WB). GTX17259

GTX17259 WB Image

WB analysis of 25 ug of Hela (lane 1), A431 (lane 2) and mouse brain (lane 3) using GTX17259 alpha Catenin antibody [1G5].
Dilution : 1:500

Anti-alpha Catenin antibody [1G5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17259

GTX17259 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX17259 alpha Catenin antibody [1G5]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-alpha Catenin antibody [1G5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17259

GTX17259 ICC/IF Image

ICC/IF analysis of A2058 Cells using GTX17259 alpha Catenin antibody [1G5]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-alpha Catenin antibody [1G5] used in Western Blot (WB). GTX17259

GTX17259 WB Image

WB analysis of whole cell extracts (30 μg lysate) of HeLa (Lane 1), A-431 (Lane 2) and MCF7 (Lane 3) using GTX17259 alpha Catenin antibody [1G5].
Dilution : 1:250-1:750

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399