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alpha Catenin antibody [N1N3]

Anti-alpha Catenin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111168
Anti-alpha Catenin antibody [N1N3] used in Immunoprecipitation (IP). GTX111168
Anti-alpha Catenin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111168
Anti-alpha Catenin antibody [N1N3] used in Western Blot (WB). GTX111168
Anti-alpha Catenin antibody [N1N3] used in Western Blot (WB). GTX111168
Anti-alpha Catenin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111168
Anti-alpha Catenin antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111168
Anti-alpha Catenin antibody [N1N3] used in Western Blot (WB). GTX111168

Cat No. GTX111168

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2 , mouse brain , Rat2

Predict Reactivity

Zebrafish, Rabbit, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.99 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human alpha 1 Catenin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Anti-alpha Catenin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111168

GTX111168 IHC-P Image

alpha Catenin antibody [N1N3] detects alpha Catenin protein at cell membrane and cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Green: alpha Catenin antibody [N1N3] (GTX111168) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Blue: Nuclear staining with Hoechst 33342.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Catenin antibody [N1N3] used in Immunoprecipitation (IP). GTX111168

GTX111168 IP Image

alpha 1 Catenin antibody [N1N3] immunoprecipitates CTNNA1 protein in IP experiments.
IP samples: HeLa whole cell extract
A. 30 μg HeLa whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of CTNNA1 protein by 4 μg alpha 1 Catenin antibody [N1N3] (GTX111168)
5 % SDS-PAGE
The immunoprecipitated CTNNA1 protein was detected by alpha 1 Catenin antibody [N1N3] (GTX111168) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Anti-alpha Catenin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111168

GTX111168 IHC-P Image

alpha 1 Catenin antibody [N1N3] detects alpha 1 Catenin protein at membrane on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
alpha 1 Catenin antibody [N1N3] (GTX111168) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha Catenin antibody [N1N3] used in Western Blot (WB). GTX111168

GTX111168 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX111168 diluted at 1:1000

Anti-alpha Catenin antibody [N1N3] used in Western Blot (WB). GTX111168

GTX111168 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Catenin antibody [N1N3] (GTX111168) diluted at 1:500.

Anti-alpha Catenin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111168

GTX111168 IHC-P Image

alpha 1 Catenin antibody [N1N3] detects alpha 1 Catenin protein at membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
alpha 1 Catenin antibody [N1N3] (GTX111168) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha Catenin antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111168

GTX111168 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using alpha Catenin(GTX111168) antibody at 1:500 dilution.

Anti-alpha Catenin antibody [N1N3] used in Western Blot (WB). GTX111168

GTX111168 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: Hela
7.5% SDS PAGE
GTX111168 diluted at 1:1000

REFERENCE
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REVIEW

alpha Catenin antibody [N1N3] Cat No. GTX111168

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 23-Aug-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : HUVEC cells
Amount used : 20μg
Blocking : 5% milk in PBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169