GeneTex
United States (US)

alpha Catenin antibody [N1N3]

GTX111168_40079_20150408_IHC-P_M_19011507_250.jpg
GTX111168_40079_IP_19011507_760.jpg
GTX111168_40079_20141017_IHC_M_19011507_971.jpg
GTX111168_40079_WB_M_brain_19011507_598.jpg
GTX111168_40079_20160310_WB_R_19011507_430.jpg
GTX111168_40079_20141017_IHC_M_2_19011507_171.jpg
GTX111168_40079_IFA_19011507_612.jpg
GTX111168_WB_19011507_359.jpg

Cat No. GTX111168

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2 , mouse brain , Rat2

Predict Reactivity

Zebrafish, Rabbit, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Concentration

0.99mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human alpha 1 Catenin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
GTX111168_40079_20150408_IHC-P_M_19011507_250.jpg

GTX111168 IHC-P Image

alpha Catenin antibody [N1N3] detects alpha Catenin protein at cell membrane and cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Green: alpha Catenin antibody [N1N3] (GTX111168) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Blue: Nuclear staining with Hoechst 33342.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX111168_40079_IP_19011507_760.jpg

GTX111168 IP Image

alpha 1 Catenin antibody [N1N3] immunoprecipitates CTNNA1 protein in IP experiments.
IP samples: HeLa whole cell extract
A. 30 μg HeLa whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of CTNNA1 protein by 4 μg alpha 1 Catenin antibody [N1N3] (GTX111168)
5 % SDS-PAGE
The immunoprecipitated CTNNA1 protein was detected by alpha 1 Catenin antibody [N1N3] (GTX111168) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX111168_40079_20141017_IHC_M_19011507_971.jpg

GTX111168 IHC-P Image

alpha 1 Catenin antibody [N1N3] detects alpha 1 Catenin protein at membrane on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
alpha 1 Catenin antibody [N1N3] (GTX111168) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX111168_40079_WB_M_brain_19011507_598.jpg

GTX111168 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX111168 diluted at 1:1000

GTX111168_40079_20160310_WB_R_19011507_430.jpg

GTX111168 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Catenin antibody [N1N3] (GTX111168) diluted at 1:500.

GTX111168_40079_20141017_IHC_M_2_19011507_171.jpg

GTX111168 IHC-P Image

alpha 1 Catenin antibody [N1N3] detects alpha 1 Catenin protein at membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
alpha 1 Catenin antibody [N1N3] (GTX111168) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX111168_40079_IFA_19011507_612.jpg

GTX111168 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using alpha Catenin(GTX111168) antibody at 1:500 dilution.

GTX111168_WB_19011507_359.jpg

GTX111168 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: Hela
7.5% SDS PAGE
GTX111168 diluted at 1:1000

REVIEW

alpha Catenin antibody [N1N3] Cat No. GTX111168

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 23-Aug-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : HUVEC cells
Amount used : 20μg
Blocking : 5% milk in PBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
SDS
Glycerol.pdf
Thimerosal.pdf