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alpha Catenin antibody [N1N3]

Anti-alpha Catenin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111168
Anti-alpha Catenin antibody [N1N3] used in Immunoprecipitation (IP). GTX111168
Anti-alpha Catenin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111168
Anti-alpha Catenin antibody [N1N3] used in Western Blot (WB). GTX111168
Anti-alpha Catenin antibody [N1N3] used in Western Blot (WB). GTX111168
Anti-alpha Catenin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111168
Anti-alpha Catenin antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111168
Anti-alpha Catenin antibody [N1N3] used in Western Blot (WB). GTX111168

Cat No. GTX111168

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2 , mouse brain , Rat2

Predict Reactivity

Zebrafish, Rabbit, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.99 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human alpha Catenin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950057

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-alpha Catenin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111168

GTX111168 IHC-P Image

alpha Catenin antibody [N1N3] detects alpha Catenin protein at cell membrane and cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Green: alpha Catenin antibody [N1N3] (GTX111168) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Blue: Nuclear staining with Hoechst 33342.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Catenin antibody [N1N3] used in Immunoprecipitation (IP). GTX111168

GTX111168 IP Image

alpha 1 Catenin antibody [N1N3] immunoprecipitates CTNNA1 protein in IP experiments.
IP samples: HeLa whole cell extract
A. 30 μg HeLa whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of CTNNA1 protein by 4 μg alpha 1 Catenin antibody [N1N3] (GTX111168)
5 % SDS-PAGE
The immunoprecipitated CTNNA1 protein was detected by alpha 1 Catenin antibody [N1N3] (GTX111168) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Anti-alpha Catenin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111168

GTX111168 IHC-P Image

alpha 1 Catenin antibody [N1N3] detects alpha 1 Catenin protein at membrane on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
alpha 1 Catenin antibody [N1N3] (GTX111168) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha Catenin antibody [N1N3] used in Western Blot (WB). GTX111168

GTX111168 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX111168 diluted at 1:1000

Anti-alpha Catenin antibody [N1N3] used in Western Blot (WB). GTX111168

GTX111168 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Catenin antibody [N1N3] (GTX111168) diluted at 1:500.

Anti-alpha Catenin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111168

GTX111168 IHC-P Image

alpha 1 Catenin antibody [N1N3] detects alpha 1 Catenin protein at membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
alpha 1 Catenin antibody [N1N3] (GTX111168) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha Catenin antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111168

GTX111168 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using alpha Catenin(GTX111168) antibody at 1:500 dilution.

Anti-alpha Catenin antibody [N1N3] used in Western Blot (WB). GTX111168

GTX111168 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: Hela
7.5% SDS PAGE
GTX111168 diluted at 1:1000

REFERENCE

REVIEW

alpha Catenin antibody [N1N3] Cat No. GTX111168

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 23-Aug-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : HUVEC cells
Amount used : 20μg
Blocking : 5% milk in PBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169