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alpha Fodrin antibody [D8B7]

Cat. No. GTX11755

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 D8B7

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-Fr, ELISA

Reactivity

 Human
Package
100 μl ($429)
See all alpha Fodrin products

APPLICATION

Application Note

ELISA: Use at a dilution of 1/10^5-1/10^6IF: Use at a dilution of 1/100 - 1/500. IHC-Fr: Use at a dilution of 1/50 - 1/100. WB: Use at a dilution of 1/10^2-1/10^3. Predicted molecular weight: 297 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

285 kDa. ( Note )

Product Note

Clone D8B7 is developed against the alpha II spectrin SH3 domain (Xu et al., 2001). Clone D8B7 is a specific marker for human, rat and mouse alpha II spectrin or fodrin. In cerebellar cell cultures, it labelsaxons and periphery of cell bodies consistent with localization of fodrin(reviewed in Bennett, 1993; Goodman, 1995 & De Matteis, 2000). It does notcrossreact with alpha I (erythroid) spectrin SH3 domain.

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant alpha II spectrin (non-erythroid spectrin or fodrin) SH3 domain (Human).

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

spectrin alpha, non-erythrocytic 1 , EIEE5 , NEAS , SPTA2

Cellular Localization

Cytoplasm, cytoskeleton

Background

Spectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. Spectrins are composed of alpha and beta dimers that associate to form tetramers linked in a head-to-head arrangement. This gene encodes an alpha spectrin that is specifically expressed in nonerythrocytic cells. The encoded protein has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in this gene are the cause of early infantile epileptic encephalopathy-5. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010]

Database

Research Area

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 429