alpha Fodrin antibody [GT1261]

Cat. No. GTX02858

Host	Rabbit
Clonality	Monoclonal
Clone Name	GT1261
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($419)

See all alpha Fodrin products

Datasheet

View product citations for antibody GTX02858 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500 - 1:2000
IHC-P	1:50 - 1:200

Not tested in other applications.

Calculated MW

285 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Alpha Fodrin

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

spectrin alpha, non-erythrocytic 1 , EIEE5 , NEAS , SPTA2

Cellular Localization

Cytoplasm, cytoskeleton

Background

Spectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. Spectrins are composed of alpha and beta dimers that associate to form tetramers linked in a head-to-head arrangement. This gene encodes an alpha spectrin that is specifically expressed in nonerythrocytic cells. The encoded protein has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in this gene are the cause of early infantile epileptic encephalopathy-5. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010]

Database

Gene ID: 6709 SPTAN1
UniProt: Q13813 SPTAN1

Research Area

DATA IMAGES

GTX02858 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with alpha Fodrin antibody [GT1261] (GTX02858) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX02858 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with alpha Fodrin antibody [GT1261] (GTX02858) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX02858 WB Image

WB analysis of HeLa whole cell lysate using GTX02858 alpha Fodrin antibody [GT1261].
Dilution : 1:1000
Loading : 25μg

GTX02858 WB Image

WB analysis of various samples using GTX02858 alpha Fodrin antibody [GT1261].
Dilution : 1:1000
Loading : 25μg

GTX02858 IHC-P Image

IHC-P analysis of mouse kidney tissue section using GTX02858 alpha Fodrin antibody [GT1261].
Dilution : 1:100

GTX02858 IHC-P Image

IHC-P analysis of human liver tissue section using GTX02858 alpha Fodrin antibody [GT1261].
Dilution : 1:100

GTX02858 IHC-P Image

IHC-P analysis of rat kidney tissue section using GTX02858 alpha Fodrin antibody [GT1261].
Dilution : 1:100

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 419

Qty

alpha Fodrin antibody [GT1261]

Cat. No. GTX02858

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX02858 WB Image

GTX02858 WB Image

GTX02858 WB Image

GTX02858 WB Image

GTX02858 IHC-P Image

GTX02858 IHC-P Image

GTX02858 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.