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alpha Fodrin antibody

Anti-alpha Fodrin antibody used in Western Blot (WB). GTX66033
Anti-alpha Fodrin antibody used in IHC (Paraffin sections) (IHC-P). GTX66033
Anti-alpha Fodrin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66033
Anti-alpha Fodrin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66033
Anti-alpha Fodrin antibody used in IHC (Paraffin sections) (IHC-P). GTX66033
Anti-alpha Fodrin antibody used in IHC (Paraffin sections) (IHC-P). GTX66033

Cat. No. GTX66033

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all alpha Fodrin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

285 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 950-1130 of human SPTAN1 (NP_001123910.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

spectrin alpha, non-erythrocytic 1 , EIEE5 , NEAS , SPTA2

Cellular Localization

Cytoplasm, cytoskeleton

Background

Spectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. Spectrins are composed of alpha and beta dimers that associate to form tetramers linked in a head-to-head arrangement. This gene encodes an alpha spectrin that is specifically expressed in nonerythrocytic cells. The encoded protein has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in this gene are the cause of early infantile epileptic encephalopathy-5. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010]

Database

Research Area

DATA IMAGES

Anti-alpha Fodrin antibody used in Western Blot (WB). GTX66033

GTX66033 WB Image

WB analysis of various sample lysates using GTX66033 alpha Fodrin antibody.
Dilution : 1:3000
Loading : 25μg per lane

Anti-alpha Fodrin antibody used in IHC (Paraffin sections) (IHC-P). GTX66033

GTX66033 IHC-P Image

IHC-P analysis of rat liver tissue using GTX66033 alpha Fodrin antibody.
Dilution : 1:100

Anti-alpha Fodrin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66033

GTX66033 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX66033 alpha Fodrin antibody.
Blue : DAPI
Dilution : 1:100

Anti-alpha Fodrin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66033

GTX66033 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX66033 alpha Fodrin antibody.
Blue : DAPI
Dilution : 1:100

Anti-alpha Fodrin antibody used in IHC (Paraffin sections) (IHC-P). GTX66033

GTX66033 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX66033 alpha Fodrin antibody.
Dilution : 1:100

Anti-alpha Fodrin antibody used in IHC (Paraffin sections) (IHC-P). GTX66033

GTX66033 IHC-P Image

IHC-P analysis of human stomach tissue using GTX66033 alpha Fodrin antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399