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alpha Fodrin (cleaved Asp1185) antibody

Anti-alpha Fodrin (cleaved Asp1185) antibody used in Western Blot (WB). GTX86886

Cat. No. GTX86886

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μg ($399)
See all alpha Fodrin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
Not tested in other applications.

Calculated MW

285 kDa. ( Note )

Product Note

alpha Fodrin (cleaved Asp1185) antibody detects endogenous levels of fragment of activated alpha Fodrin / SPTA2 resulting from cleavage adjacent to Asp1185.

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human alpha Fodrin (1136-1185).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

EIEE5 , NEAS , SPTA2 , SPTAN1 , spectrin alpha, nonerythrocytic 1 , spectrin α II , alpha Fodrin , alpha-II spectrin , spectrin alpha, non-erythrocytic 1

Cellular Localization

Cytoplasm,Cytoskeleton,Cytoplasm,Cell cortex

Background

Spectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. Spectrins are composed of alpha and beta dimers that associate to form tetramers linked in a head-to-head arrangement. This gene encodes an alpha spectrin that is specifically expressed in nonerythrocytic cells. The encoded protein has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in this gene are the cause of early infantile epileptic encephalopathy-5. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010]

Database

Research Area

DATA IMAGES

Anti-alpha Fodrin (cleaved Asp1185) antibody used in Western Blot (WB). GTX86886

GTX86886 WB Image

WB analysis of HeLa and A549 cells treated with etoposide 25uM (24h) lysate using GTX86886 alpha Fodrin (cleaved Asp1185) antibody. The lane on the right is blocked with the synthesized peptide.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399