GeneTex
United States (US)

alpha Glucosidase II antibody [N1N2], N-term

KO/KD
GTX102237_39995_20181102_WB_KO_watermark_19011519_941.jpg
GTX102237_WB_19011506_525.jpg
GTX102237_IHC_19011506_757.jpg
GTX102237_39995_20151223_IHC-P_19011506_253.jpg
GTX102237_39995_WB_M_brain_19011506_969.jpg
GTX102237_39995_IFA_19011506_457.jpg

Cat No. GTX102237

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

107 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human alpha Glucosidase II. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

G2AN antibody, GLUII antibody, KIAA0088 antibody, GANAB antibody, alpha-glucosidase 2 antibody, glucosidase II subunit alpha antibody, neutral alpha-glucosidase AB antibody, alpha glucosidase II alpha subunit antibody, glucosidase, alpha, neutral AB antibody

Cellular Localization

Endoplasmic reticulum (By similarity) , Golgi apparatus (By similarity) , Melanosome

Database

DATA IMAGES
GTX102237_39995_20181102_WB_KO_watermark_19011519_941.jpg

GTX102237 WB Image

KO/KD

Wild-type (WT) and alpha Glucosidase II knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Glucosidase II antibody [N1N2], N-term (GTX102237) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX102237_WB_19011506_525.jpg

GTX102237 WB Image

Sample(30 µg whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: HeLa S3 (GTX14654)
E: HepG2 (GTX27900)
F: MOLT4 (GTX27912)
G: Raji (GTX27908)
7.5% SDS PAGE
GTX102237 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102237_IHC_19011506_757.jpg

GTX102237 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using alpha Glucosidase II(GTX102237) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102237_39995_20151223_IHC-P_19011506_253.jpg

GTX102237 IHC-P Image

alpha Glucosidase II antibody [N1N2], N-term detects alpha Glucosidase II protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
alpha Glucosidase II antibody [N1N2], N-term (GTX102237) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102237_39995_WB_M_brain_19011506_969.jpg

GTX102237 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX102237 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102237_39995_IFA_19011506_457.jpg

GTX102237 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using alpha Glucosidase II(GTX102237) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169