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alpha Glucosidase II antibody [N1N2], N-term

Anti-alpha Glucosidase II antibody [N1N2], N-term used in Western Blot (WB). GTX102237
Anti-alpha Glucosidase II antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102237
Anti-alpha Glucosidase II antibody [N1N2], N-term used in Western Blot (WB). GTX102237
Anti-alpha Glucosidase II antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102237
Anti-alpha Glucosidase II antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102237
Anti-alpha Glucosidase II antibody [N1N2], N-term used in Western Blot (WB). GTX102237

Cat No. GTX102237

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

107 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human alpha Glucosidase II. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950338

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glucosidase II alpha subunit , G2AN , GIIA , GLUII , PKD3

Cellular Localization

Endoplasmic reticulum , Golgi apparatus , Melanosome

Database

Research Area

DATA IMAGES

Anti-alpha Glucosidase II antibody [N1N2], N-term used in Western Blot (WB). GTX102237

GTX102237 WB Image

Wild-type (WT) and alpha Glucosidase II knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Glucosidase II antibody [N1N2], N-term (GTX102237) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Glucosidase II antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102237

GTX102237 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using alpha Glucosidase II(GTX102237) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-alpha Glucosidase II antibody [N1N2], N-term used in Western Blot (WB). GTX102237

GTX102237 WB Image

Sample(30 μg whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: HeLa S3 (GTX14654)
E: HepG2 (GTX27900)
F: MOLT4 (GTX27912)
G: Raji (GTX27908)
7.5% SDS PAGE
GTX102237 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Glucosidase II antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102237

GTX102237 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using alpha Glucosidase II(GTX102237) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Glucosidase II antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102237

GTX102237 IHC-P Image

alpha Glucosidase II antibody [N1N2], N-term detects alpha Glucosidase II protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
alpha Glucosidase II antibody [N1N2], N-term (GTX102237) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Glucosidase II antibody [N1N2], N-term used in Western Blot (WB). GTX102237

GTX102237 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX102237 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

alpha Glucosidase II antibody [N1N2], N-term Cat No. GTX102237

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2016.PMID: 27283361
SDS
Glycerol.pdf
Thimerosal.pdf