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alpha Internexin antibody

Anti-alpha Internexin antibody used in Western Blot (WB). GTX130053
Anti-alpha Internexin antibody used in Immunoprecipitation (IP). GTX130053
Anti-alpha Internexin antibody used in Western Blot (WB). GTX130053
Anti-alpha Internexin antibody used in Western Blot (WB). GTX130053
Anti-alpha Internexin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130053
Anti-alpha Internexin antibody used in Western Blot (WB). GTX130053

Cat No. GTX130053

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:800
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , SK-N-AS , SH-SY5Y , mouse brain , rat brain

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human alpha Internexin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

internexin neuronal intermediate filament protein alpha , NEF5 , NF-66 , TXBP-1

Background

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene is a member of the intermediate filament family and is involved in the morphogenesis of neurons. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-alpha Internexin antibody used in Western Blot (WB). GTX130053

GTX130053 WB Image

alpha Internexin antibody detects alpha Internexin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Internexin antibody (GTX130053) diluted by 1:1000.

Anti-alpha Internexin antibody used in Immunoprecipitation (IP). GTX130053

GTX130053 IP Image

Immunoprecipitation of alpha Internexin protein from U87-MG whole cell extracts using 5 μg of alpha Internexin antibody (GTX130053).
Western blot analysis was performed using alpha Internexin antibody (GTX130053) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-alpha Internexin antibody used in Western Blot (WB). GTX130053

GTX130053 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Internexin antibody (GTX130053) diluted at 1:1000.

Anti-alpha Internexin antibody used in Western Blot (WB). GTX130053

GTX130053 WB Image

alpha Internexin antibody detects alpha Internexin protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Internexin antibody (GTX130053) diluted by 1:1000.

Anti-alpha Internexin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130053

GTX130053 ICC/IF Image

alpha Internexin antibody detects alpha Internexin protein expression by immunofluorescent analysis.
Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.
Red: alpha Internexin protein stained by alpha Internexin antibody (GTX130053) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-alpha Internexin antibody used in Western Blot (WB). GTX130053

GTX130053 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Internexin antibody (GTX130053) diluted at 1:1000.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169